CAREER: Integrating Chemical Biology into Research and Education: Studying Glycosylation of Cellulolytic Enzymes

职业：将化学生物学融入研究和教育：研究纤维素分解酶的糖基化

• 批准号: 1454925
• 负责人: Zhongping Tan
• 金额: $ 65万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-15 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1454925&HistoricalAwards=false
• 关键词: CAREER; Integrating; Chemical; Biology; into

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Zhongping Tan from the University of Colorado at Boulder to investigate the glycosylation of cellulases, a class of important enzymes that are widely used in industry for the production of fuels derived from renewable biological materials (biofuels). In order to reduce the costs of producing biofuels, it is paramount to enhance the performance of cellulases and glycosylation, i.e., the attachment of carbohydrates to proteins, appears to be a promising way to achieve such a task. The proposed research seeks to establish a link between glycosylation and cellulase performance and provide clear guidelines to develop more efficient cellulase enzymes. By working on this project, students will gain a diverse range of skills and experiences across chemistry and biology. This project includes plans to educate elementary and secondary school science teachers on the production of renewable biofuels. Through the combined use of chemical synthesis, enzymatic biotransformation, and biochemical characterization, this research project seeks to unveil the molecular level impact of cellulase glycosylation. The proposed approach will use both chemical and enzymatic synthesis to prepare a library of homogeneous glycosylated protein isoforms (glycoforms) with control of the number of occupied glycosylation sites and of glycan composition. The effects of glycosylation on stability and function will be quantified by comparing the characteristics across these well defined glycoform arrays. This type of systematic knowledge is anticipated to not only shed light on the possible roles of glycosylation on industrially important enzymes, but also lead to the identification of cellulase glyco-variants with enhanced efficiency relative to those currently used in industrial processes.

通过该奖项，化学生命过程的化学过程计划为科罗拉多大学博尔德分校的张坦（Zhonging Tan）博士提供了研究，以研究纤维素酶的糖基化糖基化，这是一类重要酶，这些酶在行业中广泛用于生产从可再生生物材料（Biofuels）中得出的燃料。 为了降低产生生物燃料的成本，提高纤维素酶和糖基化的性能，即碳水化合物与蛋白质的附着是至关重要的，这似乎是实现此类任务的一种有希望的方法。拟议的研究旨在建立糖基化和纤维素酶性能之间的联系，并提供明确的指南来开发更有效的纤维素酶。通过从事该项目，学生将获得化学和生物学的各种技能和经验。该项目包括计划向小学和中学科学教师提供有关可再生生物燃料的生产的计划。 通过化学合成，酶促生物转化和生化特征的联合使用，该研究项目旨在揭示纤维酶糖基化的分子水平影响。所提出的方法将使用化学和酶合成来制备均质糖基化蛋白质同工型（糖型）的库，并控制占据的糖基化位点和聚糖组成的数量。糖基化对稳定性和功能的影响将通过比较这些定义良好的糖型阵列的特征来量化。预计这种系统的知识不仅可以阐明糖基化在工业重要酶中的可能作用，而且还可以鉴定出相对于目前在工业过程中使用的纤维酶的糖酶糖体变化的效率。