MRI:Acquisition of a multifunctional imager for research, undergraduate training, and curricular applications

MRI：购买多功能成像仪用于研究、本科培训和课程应用

• 批准号: 1428387
• 负责人: Janet Kirkley
• 金额: $ 4.19万
• 依托单位: Knox College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1428387&HistoricalAwards=false
• 关键词: MRI; Acquisition; multifunctional; imager; research

An award is made to Knox College to acquire a Bio-Rad V3 WW ChemiDoc MP imager package. The imager will contribute to research activities and undergraduate education in Biochemistry, Biology, and Neuroscience, permitting accelerated gel electrophoresis and transfer and fluorescence detection for Western blotting, as well as supporting multiple methods for visualization of protein and nucleic acid gels. The ChemiDoc MP will contribute significantly to undergraduate research and curricular applications, thus enhancing student research training at Knox. Incorporation of the instrument into coursework and laboratory activities in Biochemistry, Biology, and Neuroscience will benefit approximately 140 students per year. The instrument will allow for adoption of more environmentally-friendly techniques and will improve data acquisition and analysis.Because of the broad application of the techniques supported by this instrument, the ChemiDoc MP imager will contribute to research in a number of laboratories. Research into the mechanisms by which ethanol decreases the immune defense will use the imager to study changes in signal transduction events occurring early in the inflammatory response to better understand how acute ethanol exposure impairs defense against illness-producing pathogens. The instrument will also be used for research addressing the role of intersectin in cellular transport of macromolecules during the development of the nervous system, with implications for better understanding crucial events in early development. Another research program uses molecular biology techniques facilitated by the imager to create a more robust protein-based hydrogel biopolymer, which has important uses in tissue engineering and drug delivery design. One laboratory will visualize nucleic acid gels as part of its exploration of the diversity of small RNAs expressed in dictyostelid social amoebas as a way of achieving better understanding of the evolutionary history of these important regulators of gene expression. The imager will also be used in research that addresses gender differences in the development of drug addiction by studying the role of the female hormone estrogen in the development of drug dependence. These programs combined will involve five investigators and approximately 20 undergraduate students annually in independent research initiatives.

为诺克斯学院颁发了奖励，以获取Bio-Rad V3 WW Chemidoc MP Imager包。 该成像仪将在生物化学，生物学和神经科学领域有助于研究活动和本科教育，允许加速凝胶电泳以及转移和荧光检测，以进行蛋白质印迹，并支持多种可视化蛋白质和核酸凝胶的方法。 Chemidoc MP将为本科研究和课程应用做出重大贡献，从而增强了Knox的学生研究培训。 将仪器纳入生物化学，生物学和神经科学的课程和实验室活动中，每年将使大约140名学生受益。 该工具将允许采用更环保的技术，并将改善数据获取和分析。由于该工具支持的技术广泛应用，Chemidoc MP成像器将有助于许多实验室的研究。 研究乙醇减少免疫防御的机制将使用成像仪研究炎症反应早期发生的信号转导事件的变化，以更好地了解急性乙醇暴露如何损害防御疾病产生病原体的防御。该仪器还将用于研究，以解决神经系统发育过程中索塞蛋白在大分子的细胞转运中的作用，这对更好地理解早期发育中的关键事件的意义。另一项研究计划使用成像仪促进的分子生物学技术来创建一个更健壮的基于蛋白质的水凝胶生物聚合物，该技术在组织工程和药物递送设计中具有重要用途。一个实验室将可视化核酸凝胶，以此作为其对dictyostelid社会变形虫表达的小RNA多样性的探索的一部分，作为一种更好地理解基因表达的这些重要调节剂的进化历史的一种方式。该成像仪还将通过研究女性激素雌激素在药物依赖性发展中的作用来解决药物成瘾发展中性别差异的研究。 这些计划的合并将涉及五名调查人员，每年大约有20名本科生参加独立的研究计划。