CNIC: U.S.-Netherlands Project Development for Research on the Zebrafish (Danio rerio) as a Toxicological Model Using Magnetic Resonance Spectroscopy and Imaging Techniques

CNIC：美国-荷兰利用磁共振波谱和成像技术研究斑马鱼（Danio rerio）毒理学模型的项目开发

• 批准号: 1427797
• 负责人: John Berry
• 金额: $ 5.76万
• 依托单位: Florida International University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1427797&HistoricalAwards=false
• 关键词: CNIC; U.S.; Netherlands; Project; Development

This research visit will initiate a new collaboration between the P.I., Dr. John Berry, at Florida International University, and counterparts at laboratories in the Netherlands and Germany. Their goal is to develop cutting-edge techniques - based on principles of magnetic resonance, and the zebrafish as an aquatic animal model - for investigation of environmental toxicants, and specifically toxic metabolites derived from marine and freshwater "harmful algal bloom" (HAB) species. Aquatic animals, as "model systems," are fundamental tools to understanding environmental toxicants. Of these, the zebrafish (Danio rerio) has emerged as an important toxicological model for use in a wide range of relevant toxic compounds in the environment, including those associated with HABs. Magnetic resonance (MR) utilizes the inherent "magnetic spin" of atomic nuclei to selectively distinguish molecules of interest. When applied to living systems, MR is capable of high-resolution imaging as well as probing the uptake, fate and metabolism of molecules. The laboratory of the international partner, Dr. A. Alia, jointly housed at the University of Leiden, in the Netherlands, and at Leipzig University, in Germany, has recently developed MR imaging and spectroscopy techniques that are capable of visualization/imaging and quantifying metabolites in the zebrafish, including embryo, larval and adult stages. Accordingly, the plans for initiating this research will require obtaining initial data demonstrating the use of MR to investigate uptake, localization, and metabolism of several relevant HAB toxins in the zebrafish model. Finally, although this research planning visit by the P.I. and one U.S. graduate student will focus specifically on investigation of HAB toxins in the zebrafish system, the techniques to be jointly developed in the future have the potential to be transformative in the field of environmental toxicology, and if successful, the proposed approach may be applied to a wide range of environmental contaminants, such as industrial pollutants, oil spills, or toxic metals.

这次研究访问将在佛罗里达国际大学的P.I.，John Berry博士与荷兰和德国实验室的同行之间进行新的合作。 他们的目标是根据磁共振原理和作为水生动物模型的斑马鱼开发最先进的技术，以研究环境有毒物质，特别是源自海洋和淡水“有害的藻类开花”（HAB）的有毒代谢物（HAB）。 水生动物作为“模型系统”是了解环境有毒物质的基本工具。 其中，斑马鱼（Danio Rerio）已成为一种重要的毒理学模型，用于在环境中的广泛相关毒性化合物中使用，包括与HAB相关的毒物。 磁共振（MR）利用原子核的固有“磁性自旋”选择性区分了感兴趣的分子。当应用于生活系统时，MR能够进行高分辨率成像，并探测分子的摄取，命运和代谢。 国际合作伙伴A. Alia博士的实验室联合住在荷兰的莱顿大学和德国的莱比锡大学，最近开发了能够可视化/成像和量化Zebrafish的代谢物的MR成像和光谱技术。 因此，启动这项研究的计划将需要获取初始数据，以证明MR在斑马鱼模型中使用MR来研究几种相关HAB毒素的摄取，定位和代谢。 最后，尽管P.I.的研究计划访问而且，一位美国研究生将专门针对斑马鱼系统中HAB毒素的调查，将来要共同开发的技术具有在环境毒理学领域进行变革的潜力，如果成功，则建议的方法可应用于各种环境污染物，例如各种环境污染物，例如工业污染物，油性漏油，油性泡沫，或有毒的Metals。