Collaborative Research: Plug and Play Photosynthesis for RuBisCO Independent Fuels

合作研究：RuBisCO 独立燃料的即插即用光合作用

• 批准号: 1359575
• 负责人: Ichiro Matsumura
• 金额: $ 41.56万
• 依托单位: Emory University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1359575&HistoricalAwards=false
• 关键词: Collaborative; Research; Plug; Play; Photosynthesis

Global human energy consumption is expected to increase by over 30% in the next 15 years. Although there is more than enough energy in sunlight to meet this challenge, efficient means to concentrate this diffuse solar energy and store it have not yet been developed. Photosynthesis is the biological process by which green plants and some microorganisms capture and store solar energy; they use it to convert carbon dioxide to metabolic fuels. However, photosynthesis is inherently an inefficient process; it is limited not by the availability of light from the sun but by the rate of the first step in the chemical reactions by which carbon dioxide is converted into products that are rich in energy. There is therefore unused capacity for the capture and use of solar energy by natural photosynthesis. This program engages teams of researchers from the US and the United Kingdom with the goal to re-invent photosynthesis with enhanced efficiency thereby improving the capacity of photosynthetic organisms to make renewable fuels and enhance food security. In the course of the scientific research, this project will provide research training opportunities for more than 14 people (including both women and underrepresented minorities) at the post-doctoral, graduate and undergraduate levels. The international and interdisciplinary nature of the project will build bridges between the US and UK scientific communities in a range of important scientific areas including synthetic biology, photosynthetic physiology, catalysis, and metabolic regulation. The researchers will engage and inform both the public and other investigators through activities that include peer-reviewed publications, public science lectures, blogs, websites, and popular science articles. To dramatically enhance the efficiency of photosynthesis, this project will develop a range of mechanisms to electrically connect light-activated electron flow from the photosynthetic reaction center (PSI) to downstream fuel-production pathways. This will include increasing flux through natural pathways, creating electrical connections between distinct microbial cell types by construction of artificial biological nanowires, and employing a soluble, chemical, redox shuttle to transfer reducing equivalents from a light harvesting cell to different fuel-producing cells. These scientific goals will be accomplished through four parallel specific aims. 1. Characterization of the components of (and mechanism for controlling flux through) the natural extracellular electron transfer pathway of the cyanobacterium Synechocystis. 2. Construction of artificial systems to abstract reducing equivalents from PSI; these will compete with natural electron acceptors only under highly reducing conditions. 3. Development of artificial means to move reducing equivalents out of the cytoplasm of Synechocystis. 4. Construction within microbes of artificial fuel-production modules that require only reducing equivalents and carbon dioxide as inputs. The project is highly interdisciplinary and will employ a range of techniques including those from microbiology, molecular biology, synthetic biology, biochemistry, electrochemistry, and protein and metabolic engineering.This award is supported jointly by the Cellular Dynamics and Function Cluster in the Division of Molecular and Cellular Biosciences and by the Biotechnology, Biochemical and Biomass Engineering Program in the Division of Chemical, Bioengineering, Environmental and Transport Systems.

未来15年，全球人类能源消耗预计将增长30%以上。尽管阳光中有足够的能量来应对这一挑战，但尚未开发出集中和存储这种漫射太阳能的有效方法。光合作用是绿色植物和一些微生物捕获和储存太阳能的生物过程；他们用它来将二氧化碳转化为代谢燃料。然而，光合作用本质上是一个低效的过程。它不仅受到太阳光的可用性的限制，而且受到二氧化碳转化为富含能量的产物的化学反应的第一步的速率的限制。因此，通过自然光合作用捕获和使用太阳能的能力未被利用。该计划由来自美国和英国的研究团队参与，其目标是重新发明光合作用，提高效率，从而提高光合生物制造可再生燃料和增强粮食安全的能力。在科学研究过程中，该项目将为超过14人（包括女性和代表性不足的少数民族）提供博士后、研究生和本科生级别的研究培训机会。该项目的国际性和跨学科性质将在美国和英国科学界之间在合成生物学、光合生理学、催化和代谢调节等一系列重要科学领域建立桥梁。研究人员将通过同行评审出版物、公共科学讲座、博客、网站和科普文章等活动吸引公众和其他研究人员并为其提供信息。为了显着提高光合作用的效率，该项目将开发一系列机制，将光激活电子流从光合反应中心（PSI）电连接到下游燃料生产途径。这将包括增加通过自然途径的通量，通过构建人造生物纳米线在不同微生物细胞类型之间建立电连接，以及采用可溶性化学氧化还原梭将还原当量从光捕获电池转移到不同的燃料生产电池。这些科学目标将通过四个平行的具体目标来实现。 1. 蓝藻集胞藻天然细胞外电子传递途径的成分（以及控制通量的机制）的表征。 2. 构建人工系统，从 PSI 中抽象出约简等价物；它们只有在高度还原条件下才会与天然电子受体竞争。 3.开发将还原当量移出集胞藻细胞质的人工方法。 4. 在微生物内构建人工燃料生产模块，仅需要还原当量和二氧化碳作为输入。该项目是高度跨学科的，将采用一系列技术，包括微生物学、分子生物学、合成生物学、生物化学、电化学以及蛋白质和代谢工程。该奖项由分子部门细胞动力学和功能集群联合支持和细胞生物科学以及化学、生物工程、环境和运输系统部门的生物技术、生物化学和生物质工程项目。