DISSERTATION RESEARCH: Invasion phylogeography of Borrelia burgdorferi, a tick-borne pathogen

论文研究：蜱传病原体伯氏疏螺旋体的入侵系统发育地理学

• 批准号: 1401143
• 负责人: Maria Diuk-Wasser
• 金额: $ 2.11万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1401143&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Invasion; phylogeography; Borrelia; DISSERTATION; RESEARCH; Invasion; phylogeography; Borrelia

Tick-borne diseases pose a significant and growing global health burden due to the expanding range of tick vectors, reservoir hosts, and pathogens, as well as a suite of other environmental pressures. Lyme disease is the most prevalent tick-borne disease in the US and the vast majority of cases occur in the Northeast. Over the past forty years, it has rapidly spread from southern New England through Maine as well as through the Upper Midwest. Despite its epidemiological importance, the source and trajectory of the ongoing invasion remains poorly described. This project harnesses the tools of next generation sequencing to reconstruct the evolutionary history of the Lyme disease bacteria, Borrelia burgdorferi. Surveying the genomic diversity of the pathogen will allow us to infer the probable origin of invasion and pathways of spread and will inform a better understanding of the environmental or ecological factors driving the emergence. Resolving the invasion history of B. burgdorferi will inform models of continued spatial spread and targeted control measures for this important human pathogen. Further, this project will support the research of a team of three female scientists and the training of at least four undergraduate assistants, including women and minorities, and several high school students in field ecology, epidemiology, and evolutionary genomics techniques.

由于壁虱媒介，水库宿主和病原体以及其他环境压力的范围不断扩大，tick传播的疾病造成了巨大的全球健康负担。 莱姆病是美国最普遍的壁虱传播疾病，绝大多数病例发生在东北。 在过去的四十年中，它已经从新英格兰南部迅速传播到缅因州以及中西部上部。 尽管具有流行病学的重要性，但正在进行的入侵的来源和轨迹仍然描述得很差。 该项目利用下一代测序的工具来重建莱姆病细菌的进化史，博尔格多菲利（Borrelia Burgdorferi）。 调查病原体的基因组多样性将使我们能够推断出入侵和传播途径的可能起源，并可以更好地了解推动出现的环境或生态因素。解决B. burgdorferi的入侵历史将为这种重要的人类病原体的持续空间扩散和靶向控制措施提供信息。 此外，该项目将支持由三名女科学家组成的团队的研究，并培训至少四名本科助理，包括妇女和少数民族，以及几位现场生态学，流行病学和进化基因组学技术领域的高中生。