RUI: Collaborative: unPAK: undergraduates Phenotyping Arabidopsis Knockouts: A distributed genomic approach to examine evolutionarily important traits

RUI：协作：unPAK：本科生拟南芥表型敲除：检查进化重要性状的分布式基因组方法

• 批准号: 1354771
• 负责人: Jennifer Thompson
• 金额: $ 28.7万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1354771&HistoricalAwards=false
• 关键词: RUI; Collaborative; unPAK; undergraduates; Phenotyping

The unPAK (undergraduates Phenotyping Arabidopsis Knockouts) project uses the plant Arabidopsis thaliana to examine how and when differences in DNA sequences lead to differences in traits related to survival, growth, and reproduction. It will detect effects, if any, of mutation in a comprehensive collection of mutants generated by the Salk Institute, and will then compile results in a comprehensive database. To examine the interaction of genetic and environmental effects, unPAK's experiments are replicated within and across multiple growth facilities and labs, and a subset of experiments deliberately manipulate soil factors, moisture, and temperature to see how these important ecological factors interact with genetics. unPAK will survey enough mutant lines to reach coverage of a third of the A. thaliana genome (approximately 9,000 genes). All data will be uploaded into a public database at arabidopsisunpak.org, and this database of observed traits is linked to existing genomic databases. Evidence produced by unPAK will enable testing central hypotheses about the relationship between genomic features and important plant characteristics. For example, the data can be used to test whether the effects of mutation are predicted by gene attributes such as molecular signatures of selection in the past, gene function, gene family size, or by patterns of variability in natural populations.unPAK is centered around participation of undergraduate researchers in the lab and the classroom, educating and training undergraduates integratively in genetics, ecology, evolution, and bioinformatics. The network extends across diverse post-secondary institutions, with over 100 undergraduate apprentices participating across 13 laboratories in 3 years. Greater than 20 course-based undergraduate research experiences (CUREs) will be supported by the program, reaching over 600 students. Any discoveries about the influence of particular genes on fruit production, survival, or life history will have significant broader impacts in potential application to crop species.As a final part of the project the unPAK research network itself is being studied. Using mixed-methods approaches from the social sciences, researchers are documenting interactions among network participants to analyze changes in network structure over time, and to investigate factors that influence student participation and outcomes, including students' accrual of human, cultural, and social capital.

UNPAK（本科生表型拟南芥敲除）项目使用植物拟南芥来检查DNA序列中的差异以及何时导致与生存，生长和繁殖有关的性状差异。 它将检测到Salk Institute产生的全面集合突变体中突变的效果，然后在全面的数据库中编译结果。为了检查遗传和环境影响的相互作用，UNPAK的实验在多个生长设施和实验室内部和跨多个生长设施和实验室进行了复制，并且一部分实验故意操纵土壤因子，水分和温度，以了解这些重要的生态因素如何与遗传学相互作用。 UNPAK将调查足够的突变线，以达到三分之一的A. thaliana基因组（约9,000个基因）。所有数据都将上传到airidopsisunpak.org上的公共数据库中，并且该观察到的性状数据库链接到现有的基因组数据库。 UNPAK产生的证据将实现有关基因组特征与重要植物特征之间关系的中心假设。 For example, the data can be used to test whether the effects of mutation are predicted by gene attributes such as molecular signatures of selection in the past, gene function, gene family size, or by patterns of variability in natural populations.unPAK is centered around participation of undergraduate researchers in the lab and the classroom, educating and training undergraduates integratively in genetics, ecology, evolution, and bioinformatics.该网络跨越了大专院校的各种机构，在3年内，有100多名本科学徒参加了13个实验室。 该计划将支持超过20多种基于课程的本科研究经验（治疗），将获得600多名学生。关于特定基因对水果生产，生存或生活史的影响的任何发现将对农作物物种的潜在应用产生更广泛的影响。作为该项目的最后一部分，正在研究UNPAK研究网络本身。使用社会科学的混合方法方法，研究人员正在记录网络参与者之间的相互作用，以分析随着时间的推移网络结构的变化，并研究影响学生参与和成果的因素，包括学生对人类，文化和社会资本的应计。