IOS: Nutrient Exchange and Regulation at the Aphid Symbiotic Interface

IOS：蚜虫共生界面的养分交换和调节

• 批准号: 1354154
• 负责人: Alexandra Wilson de Andrade
• 金额: $ 83.1万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1354154&HistoricalAwards=false
• 关键词: IOS; Nutrient; Exchange; Regulation; Aphid

Symbioses between eukaryotic hosts and bacterial consortia are fundamentally important to human health and ecosystem function. Gathering large amounts of DNA sequence data has become relatively easy and thus, models of host/microbiome function are accumulating. Critically, most models lack experimental tests. Working in a system that involves one eukaryotic host paired with a single bacterial symbiont the investigators will test models of symbiotic function to identify fundamental design principles in symbiosis. The project will exploit a very productive collaboration to further examine the nature of nutrient transport and regulation in insects; specifically, the investigators will exploit the model aphid, Acyrthosiphon pisum, and its symbiont, Buchnera aphidicola, to fully characterize the substrate and feedback regulation of transporters between the aphid and bacterium. The first objective targets testing whole genome based models of symbiotic function and regulation. The research team will transiently express transporters of sap-feeding insects in oocytes of the South African clawed frog to test genome sequence based models of symbiotic function and host/symbiont regulation. Because aphids are both a significant crop pest and excellent models for the study of symbiosis any work that increases understanding of basic aphid biology increases our ability to develop ecologically safe methods of pest control and may facilitate development of the aphid as a model for understanding the process of bacterial infection. Functional characterization of transporters in the pea aphid and related insects will facilitate comparative work in other insect systems thus benefitting the annotation of transporters in all arthropod genomes. The second objective targets bringing NSF-funded basic research into the lives of Americans through the production of six broadcast quality films about aphids, the science of symbiosis and life as a scientist. The films will be supported by development of curricular resources that target early childhood and middle school children. Finally, this project supports the research program of a mid-career female scientist.

真核宿主与细菌联盟之间的共生对人类健康和生态系统功能至关重要。 收集大量的DNA序列数据已经变得相对容易，因此，主机/微生物组函数的模型正在积累。 至关重要的是，大多数模型都缺乏实验测试。 在一个涉及一个与单个细菌共生体配对的系统的系统中工作，研究人员将测试共生功能的模型，以鉴定共生中的基本设计原理。 该项目将利用非常有生产力的合作，以进一步研究昆虫中营养运输和调节的性质；具体而言，研究人员将利用模型蚜虫Pisum及其共生体Buchnera Aphidicola，以充分表征蚜虫和细菌之间转运蛋白的反馈调节。第一个目标目标是测试基于基因组的共生功能和调节模型。研究小组将在南非爪青蛙的卵母细胞中瞬时表达液化昆虫的转运蛋白，以测试基于基因组序列的共生功能和宿主/共生体调节模型。由于蚜虫既是一种重要的农作物害虫，又是对共生研究的出色模型，任何提高对基本蚜虫生物学的理解的工作都会提高我们开发生态安全的有害生物控制方法的能力，并可能促进蚜虫作为理解细菌感染过程的模型的发展。 转运蛋白在豌豆蚜虫和相关昆虫中的功能表征将促进其他昆虫系统中的比较工作，从而使所有节肢动物基因组中转运蛋白的注释受益。 第二个目标目标是通过制作六部有关蚜虫的广播质量电影，《共生科学与科学家的生活》，将NSF资助的基础研究带入了美国人的生活。 电影将通过开发针对幼儿和中学儿童的课程资源来支持这些电影。最后，该项目支持一位职业女性科学家的研究计划。