SBIR Phase I: High Throughput Characterization of Stem Cells using Spatial Domain Stimulus Response

SBIR 第一阶段：使用空间域刺激响应对干细胞进行高通量表征

• 批准号: 1345541
• 负责人: John Collins
• 金额: $ 15万
• 依托单位: Biopico Systems
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1345541&HistoricalAwards=false
• 关键词: SBIR; Phase; Throughput; Characterization; Stem

This Small Business Innovation Research (SBIR) Phase I project proposes to develop the technical feasibility of a high throughput label-free electrical field potential marker-based characterization of stem cells. This patent-pending technology of Spatial Domain Stimulus Response (SDSR) records stimulus response from individual cells from an array of spatial electrodes and reconstruct time domain signals. This proposed high throughput characterization of stem cells using SDSR will address the unmet need of derivation of patient-specific reprogrammed somatic cells for use in studying disease development, creating therapies for diseases or disorders and developing perfect models for the cells of the human system that are harmed in the diseases. This will contribute to the goals in regenerative medicine in sorting stem cells in order to avoid the obstacles due to teratoma formation, aberrant reprogramming, and the presence of transgenes. This proposed project will be the basis for the isolation of differentiated cells from the cells that hinder graft performance without exogenous labeling or genetic modification for clinical applications. In this project, the electrical field potential recordings of differentiated cells will be compared and correlated with gene expression markers to assess the characterization.The broader impact/commercial potential of this project, if successful, will be the development of a powerful, automated, rapid, cost-effective label-free cell sorter, and overcome the barriers that prevent successful translation of stem cell biology into clinical therapy. This cell sorting can produce an unlimited supply of viable cells with improved control, portability, and reduced cost for clinical applications. This may have a significant impact for the improvement of human health and control of human diseases. For example, the system may be a potential tool for the replacement of lost progenies, remyelination and support of adjacent cells at risk in neurodegenerative disorders such as amyotrophic lateral sclerosis, Parkinson's disease, Alzheimer's diseases, Huntington's disease, stroke, traumatic lesions such as spinal cord injury, and for studying development and progression of such diseases. The global market for tissue engineering and cell therapy products for these diseases is set to more than quadruple from 2009 to 2018. Specifically, the market for stem cell research products is estimated to be $872M, and expanding through double digit growth each year, representing a significant financial opportunity.

该小企业创新研究 (SBIR) 第一阶段项目提议开发基于高通量无标记电场电位标记的干细胞表征的技术可行性。这项正在申请专利的空间域刺激响应 (SDSR) 技术通过空间电极阵列记录单个细胞的刺激响应并重建时域信号。这项使用SDSR对干细胞进行高通量表征的提议将解决衍生患者特异性重编程体细胞的未满足需求，用于研究疾病发展、创建疾病或病症的疗法以及开发人类系统细胞的完美模型。于疾病所害。这将有助于实现再生医学分选干细胞的目标，以避免畸胎瘤形成、异常重编程和转基因存在造成的障碍。该拟议项目将成为从阻碍移植物性能的细胞中分离分化细胞的基础，无需外源标记或基因修饰即可用于临床应用。在这个项目中，分化细胞的电场电位记录将与基因表达标记进行比较和关联，以评估表征。如果成功，该项目更广泛的影响/商业潜力将是开发一个强大的、自动化的、快速的，经济高效的无标记细胞分选仪，并克服阻碍干细胞生物学成功转化为临床治疗的障碍。这种细胞分选可以产生无限量的活细胞，并具有改进的控制性、便携性并降低临床应用的成本。 这可能对改善人类健康和控制人类疾病产生重大影响。例如，该系统可能是一个潜在的工具，用于替换丢失的后代、髓鞘再生和支持神经退行性疾病（如肌萎缩性脊髓侧索硬化症、帕金森病、阿尔茨海默病、亨廷顿病、中风、创伤性病变（如脊髓损伤））中的相邻细胞。脊髓损伤，以及研究此类疾病的发展和进展。从 2009 年到 2018 年，用于这些疾病的组织工程和细胞治疗产品的全球市场预计将增长四倍以上。具体而言，干细胞研究产品的市场估计为 8.72 亿美元，并且每年以两位数的速度增长，代表一个重要的财务机会。