CAREER: The Impact of Aneuploidy on Transcription: A Mechanistic Approach

职业：非整倍体对转录的影响：一种机制方法

• 批准号: 1350915
• 负责人: Robin Dowell
• 金额: $ 67.35万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1350915&HistoricalAwards=false
• 关键词: CAREER; Impact; Aneuploidy; Transcription; Mechanistic

Aneuploidy, an abnormal chromosome copy number, is common in evolution and nature. For the cell, there are both positive and negative effects of aneuploidy. Aneuploidy alters transcription but the mechanisms driving these changes are not understood. The powerful yeast model organism will be utilized to ascertain the underlying molecular mechanism using a combination of chromatin immunoprecipitation, next-generation sequencing, and computational analysis. Understanding these mechanisms will provide tremendous insight into not only aneuploidy but also transcriptional regulation in general. This project will lead to a better understanding of the costs and benefits of aneuploidy. Broader Impact A similar cross-disciplinary approach will be utilized to build a summer undergraduate research program in synthetic biology and develop an interactive fiction tool for teaching responsible conduct of research. Tremendous leaps in scientific understanding often arise from individuals who ignore traditional disciplinary boundaries to relentlessly pursue the question at hand. In this proposal, the PI seeks to instill this philosophy into generations of undergraduates through the development of a University of Colorado internationally Genetically Engineered Machines (iGEM) team. This synthetic biology competition brings students together for an intensive summer to design and construct unique biological circuits. Additionally, the PI seeks to bring the ante-disciplinary philosophy to teaching responsible conduct of research by developing an interactive fiction game. This game will not only train scientists in an engaging and interactive manner but also will enable studies into how peer pressure influences ethical behavior. The long term impact of creating honest, intelligent, and creative scientists is incalculable.

非整倍性是一种异常的染色体拷贝数，在进化和性质上很常见。 对于细胞，非整倍性具有正面和负面影响。 非整倍性改变了转录，但驱动这些变化的机制尚不清楚。 强大的酵母模型生物将使用染色质免疫沉淀，下一代测序和计算分析的组合来确定基本的分子机制。 了解这些机制将不仅可以深入了解非整倍性，而且还将提供一般的转录调节。 该项目将更好地了解非整倍性的成本和收益。 更广泛的影响将采用类似的跨学科方法来建立合成生物学的夏季本科研究计划，并开发一种互动小说工具来教授负责任的研究。 科学理解的巨大飞跃通常是由忽略传统纪律界限的个人而产生的，以无情地追求手头的问题。 在该提案中，PI试图通过发展科罗拉多大学国际基因工程机器（IGEM）团队的发展，将这种哲学灌输到本科生中。 这场合成的生物学竞赛使学生聚集在一起，以设计和构建独特的生物电路。 此外，PI旨在通过开发互动小说游戏来将雄鹿学科的哲学带入负责任的研究。 该游戏不仅将以引人入胜且互动的方式训练科学家，而且还可以研究同伴压力如何影响道德行为。 创建诚实，聪明和创造性科学家的长期影响是无法估量的。