CAREER: The Impact of Aneuploidy on Transcription: A Mechanistic Approach

职业：非整倍体对转录的影响：一种机制方法

• 批准号: 1350915
• 负责人: Robin Dowell
• 金额: $ 67.35万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1350915&HistoricalAwards=false
• 关键词: CAREER; Impact; Aneuploidy; Transcription; Mechanistic

Aneuploidy, an abnormal chromosome copy number, is common in evolution and nature. For the cell, there are both positive and negative effects of aneuploidy. Aneuploidy alters transcription but the mechanisms driving these changes are not understood. The powerful yeast model organism will be utilized to ascertain the underlying molecular mechanism using a combination of chromatin immunoprecipitation, next-generation sequencing, and computational analysis. Understanding these mechanisms will provide tremendous insight into not only aneuploidy but also transcriptional regulation in general. This project will lead to a better understanding of the costs and benefits of aneuploidy. Broader Impact A similar cross-disciplinary approach will be utilized to build a summer undergraduate research program in synthetic biology and develop an interactive fiction tool for teaching responsible conduct of research. Tremendous leaps in scientific understanding often arise from individuals who ignore traditional disciplinary boundaries to relentlessly pursue the question at hand. In this proposal, the PI seeks to instill this philosophy into generations of undergraduates through the development of a University of Colorado internationally Genetically Engineered Machines (iGEM) team. This synthetic biology competition brings students together for an intensive summer to design and construct unique biological circuits. Additionally, the PI seeks to bring the ante-disciplinary philosophy to teaching responsible conduct of research by developing an interactive fiction game. This game will not only train scientists in an engaging and interactive manner but also will enable studies into how peer pressure influences ethical behavior. The long term impact of creating honest, intelligent, and creative scientists is incalculable.

非整倍体是一种异常的染色体拷贝数，在进化和自然界中很常见。 对于细胞来说，非整倍性既有积极的影响，也有消极的影响。 非整倍性会改变转录，但驱动这些变化的机制尚不清楚。 强大的酵母模型生物体将用于结合染色质免疫沉淀、下一代测序和计算分析来确定潜在的分子机制。 了解这些机制不仅可以深入了解非整倍性，还可以深入了解一般的转录调控。 该项目将有助于更好地了解非整倍体的成本和收益。 更广泛的影响 将采用类似的跨学科方法来建立合成生物学夏季本科研究项目，并开发交互式小说工具来教授负责任的研究行为。 科学理解的巨大飞跃往往来自那些忽视传统学科界限、不懈地追求手头问题的人。 在这项提案中，PI 试图通过发展科罗拉多大学国际基因工程机器 (iGEM) 团队，将这一理念灌输给一代又一代的本科生。 这项合成生物学竞赛让学生们齐聚一堂，度过一个紧张的夏季，设计和构建独特的生物电路。 此外，PI 还试图通过开发一款互动小说游戏，将前学科哲学带入负责任的研究行为教学中。 这个游戏不仅将以一种引人入胜和互动的方式培训科学家，而且还可以研究同伴压力如何影响道德行为。 培养诚实、聪明、富有创造力的科学家的长期影响是不可估量的。