CAREER: IDBR: Super Resolution Deep Imaging

职业：IDBR：超分辨率深度成像

• 批准号: 1350654
• 负责人: Peter Kner
• 金额: $ 60.89万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1350654&HistoricalAwards=false
• 关键词: CAREER; IDBR; Super; Resolution; Deep

An award is made to the University of Georgia to create a next generation optical microscope. This microscope will take advantage of advances in chemistry, astronomy and photonics to achieve unprecedented resolution throughout large biological samples. By combining a new microscopy approach, light sheet microscopy, with technology used to look at distant galaxies and extrasolar planets, our instrument will minimize scattering and aberrations when imaging into thick samples. Combining recent advances in photochemistry with the advanced display inside a flat screen television, the instrument will control the fluorescence to achieve resolutions below the diffraction limit. This will allow subcellular details to be studied throughout zebrafish, fruit flies, roundworms and other organisms essential to modern biology research. Examples of research that will benefit from this instrument include the study of seizures in zebrafish, neuron firing in fruit flies, eye development in mouse embryos, and cell division and differentiation in developing C. elegans roundworms. Once developed, the instrument design will be made available to researchers through an open-source model to speed its adoption. The design, software and instructions will be made available on the internet. Ultimately, we plan to offer a commercial product.By advancing biological science and improving the study of organisms that are important models for human disease, this project has the potential to improve human health. The ability of this instrument to create high-resolution fluorescence images of entire multicellular organisms will be transformative in developmental biology and other areas of biological research in which the behavior of cells throughout organisms is studied. This project will train the next generation of scientists in interdisciplinary research through the development of this novel instrument, as well as through a course in bioimaging taught by biologists, chemists and engineers. Students from different disciplines will be introduced to all aspects of bioimaging from preparation and staining of samples to construction of a microscope.This award is being made jointly by two Programs- (1) Instrument Development for Biological Research, in the Division of Biological Infrastructure (Biological Sciences Directorate), and (2) Biophotonics, in the Division of Chemical, Bioengineering, Environmental and Transport Systems (Engineering Directorate).

向佐治亚大学颁发了奖励，以创建下一代光学显微镜。该显微镜将利用化学，天文学和光子学的进步，以在整个大型生物样品中实现前所未有的分辨率。通过将新的显微镜方法与用于查看遥远星系和极性行星的技术相结合，我们的仪器将在成像成厚的样品时最小化散射和像差。该仪器将最新的光化学进展与高级显示器结合在一起，将控制荧光，以达到低于衍射极限的分辨率。这将允许在整个斑马鱼，果蝇，round虫和其他对现代生物学研究必不可少的生物体中进行细胞细节的研究。将从该仪器中受益的研究例子包括研究斑马鱼中的癫痫发作，果蝇中的神经元射击，小鼠胚胎的眼睛发育以及细胞分裂以及发育中的秀丽隐杆线虫round虫的分化。一旦开发，仪器设计将通过开源模型向研究人员提供，以加快其采用。设计，软件和说明将在Internet上提供。最终，我们计划提供商业产品。通过提高生物科学并改善对人类疾病重要模型的生物的研究，该项目有可能改善人类健康。该仪器创建整个多细胞生物的高分辨率荧光图像的能力将在发育生物学和生物学研究的其他领域进行变化，其中研究了整个生物体的细胞行为。该项目将通过开发这种新颖的工具以及生物学家，化学家和工程师教授的生物成像课程来培训跨学科研究的下一代科学家。来自不同学科的学生将介绍从准备和染色到显微镜的构造的生物成像的各个方面。该奖项由两个计划共同由生物学研究工具开发，生物学基础设施（生物科学局）（生物学科学局），以及（2）生物实践局，以及（2）在化学级别的工程，环境化和环境级别的工具，以及（2）化学级别的工具。

项目成果

Stripe artifact reduction for digital scanned structured illumination light sheet microscopy

• DOI: 10.1364/ol.44.002510
• 发表时间: 2019-05-15
• 期刊: OPTICS LETTERS
• 影响因子: 3.6
• 作者: Liu, Yang;Lauderdale, James D.;Kner, Peter
• 通讯作者: Kner, Peter