EFRI-BioFlex: Cellphone-based Digital Immunoassay Platform for High-throughput Sensitive and Multiplexed Detection and Distributed Spatio-Temporal Analysis of Influenza

EFRI-BioFlex：基于手机的数字免疫分析平台，用于流感的高通量灵敏多重检测和分布式时空分析

• 批准号: 1332275
• 负责人: Aydogan Ozcan
• 金额: $ 200万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1332275&HistoricalAwards=false
• 关键词: EFRI; BioFlex; Cellphone; based; Digital

There is an urgent need for portable, rapid, sensitive and specific influenza surveillance systems worldwide. The PI proposes to fundamentally improve the diagnostic landscape of influenza within a flexible, cost-effective, and field portable telemedicine platform. They will develop a digital immunoassay system that makes use of novel electro-adaptive and programmable microfluidics technologies as well as cellphone based multispectral fluorescent cytometry and computational microscopy tools. The proposed system will create arrays of hundreds of thousands of encoded particles containing fluid droplets. These particles will be first segmented equally using inertial-microfluidic sorting so that there is a single particle per drop, where the presence of influenza subtype antigens and the associated enzyme-linked antibody sandwich will lead to an ?on or off? fluorescence signal in each drop. The number of drops lighting up (corresponding to specific color- and shape-coded particles with different subtype-specific antibodies) will then be imaged all in parallel using a cellphone-based multispectral imaging cytometry system. Through a custom-developed smart application running on the same cellphone, these immunoassay images will immediately be analyzed for sensitive and rapid detection of various influenza subtypes, where the test results will also be transmitted wirelessly to secure servers, enabling real-time spatio-temporal mapping and analysis of the disease.

全球迫切需要便携式、快速、灵敏和特异性的流感监测系统。该 PI 提议通过灵活、经济高效的现场便携式远程医疗平台从根本上改善流感的诊断环境。他们将开发一种数字免疫分析系统，该系统利用新型电自适应和可编程微流体技术以及基于手机的多光谱荧光细胞术和计算显微镜工具。所提出的系统将创建包含数十万个包含液滴的编码粒子的阵列。这些颗粒将首先使用惯性微流体分选进行均匀分割，以便每滴都有一个颗粒，其中流感亚型抗原和相关酶联抗体夹心的存在将导致“打开或关闭”。每滴中的荧光信号。然后，使用基于手机的多光谱成像细胞计数系统对点亮的液滴数量（对应于具有不同亚型特异性抗体的特定颜色和形状编码颗粒）进行并行成像。通过在同一手机上运行的定制开发的智能应用程序，这些免疫分析图像将立即被分析，以灵敏、快速地检测各种流感亚型，检测结果还将无线传输到安全服务器，从而实现实时时空检测。疾病的绘图和分析。