Developing Diagnostics for Vibrio Coralliilyticus and Coral Vibriosis

开发解珊瑚弧菌和珊瑚弧菌病的诊断方法

• 批准号: 1340665
• 负责人: Claudia Hase
• 金额: $ 6.34万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1340665&HistoricalAwards=false
• 关键词: Developing; Diagnostics; Vibrio; Coralliilyticus; Coral

This project will catalyze new collaborative research on pathogenic marine vibrios between Dr. Claudia Hase at Oregon State University, Corvallis, Oregon, and Dr. David Bourne of the Australian Institute of Marine Science (AIMS) in Townsville, Australia. The long-term goal of this collaboration is a better understanding of the role and ecology of Vibrio bacteria associated with coral disease. The research objectives of this visit are to examine the feasibility of methods developed by Dr. Hase to detect certain enzymes from Vibrio coralliilyticus. V. tubiashii has recently caused devastating losses in shellfish larvae production in the Pacific Northwest of the US and Dr. Hase has shown that a secreted metalloprotease is a critical factor for the toxicity of V. tubiashii. Dr. Bourne reported that a 99% similar enzyme of V. coralliilyticus is critical for coral bleaching and outlined the urgent need for better diagnostic tools for coral disease. Rapid and specific detection of pathogenic bacteria and their toxins in natural waters and diseased corals is paramount to better understand disease causation and to provide early warning allowing reef managers to mitigate the effect of disease outbreaks. Thus, this collaboration will benefit a broad range of marine research activities.The broader impacts of this proposed work include providing support for an OSU student to travel with the PI to AIMS and current students in the Bourne lab will participate in the proposed work. The described project provides excellent educational opportunities for students as most of the proposed experiments are straightforward and are well suited for the engagement of students in the scientific process.

该项目将促进俄勒冈州立大学（俄勒冈州科瓦利斯）的 Claudia Hase 博士和澳大利亚汤斯维尔澳大利亚海洋科学研究所 (AIMS) 的 David Bourne 博士之间关于致病性海洋弧菌的新合作研究。此次合作的长期目标是更好地了解与珊瑚疾病相关的弧菌的作用和生态。此次访问的研究目的是检验 Hase 博士开发的检测珊瑚弧菌某些酶的方法的可行性。 V. tubiashii 最近在美国西北部太平洋地区造成了贝类幼虫生产的毁灭性损失，Hase 博士表明，分泌的金属蛋白酶是 V. tubiashii 毒性的关键因素。 Bourne 博士报告称，V. Coralliilyticus 99% 相似的酶对于珊瑚白化至关重要，并概述了迫切需要更好的珊瑚疾病诊断工具。快速、具体地检测天然水域和患病珊瑚中的病原菌及其毒素对于更好地了解疾病原因并提供早期预警至关重要，使珊瑚礁管理者能够减轻疾病爆发的影响。因此，这种合作将有利于广泛的海洋研究活动。这项拟议工作的更广泛影响包括为 OSU 学生与 PI 一起前往 AIMS 提供支持，并且 Bourne 实验室的现有学生将参与拟议的工作。所述项目为学生提供了极好的教育机会，因为大多数拟议的实验都很简单，非常适合学生参与科学过程。