MRI: Acquisition of a fluorescence activated cell sorter

MRI：获取荧光激活细胞分选仪

• 批准号: 1337787
• 负责人: Dacheng Ren
• 金额: $ 45.9万
• 依托单位: Syracuse University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337787&HistoricalAwards=false
• 关键词: MRI; Acquisition; fluorescence; activated; cell

1337787RenThis proposal requests funding for the acquisition of a state-of-the-art fluorescence activated cell (FAC) sorter. Syracuse University (SU) does not currently have a FAC sorter and this is a limiting factor for many research projects in different SU departments. The selected model is a Special FACS AriaTM system with 4 lasers from BD Biosciences. It fulfills the needs of all confirmed users and has sufficient flexibility to successfully incorporate future users. It will enable researchers to sort both prokaryotic and eukaryotic cells between 0.2 - 100 ìm in diameter with high speeds (up to 70,000 cells per second) and high accuracy (99%). This major piece of advanced instrumentation will be a crucial resource for many ongoing projects at SU in the areas of biomaterials (including cell-surface interactions, drug delivery, and nanotechnology), molecular and cell biology (including neuroscience, cell physiology, development, and inter-kingdom signaling) and biofuels research involving algae cells.

1337787的提案要求资金以获取最先进的荧光激活细胞（FAC）分散器。锡拉丘兹大学（SU）目前没有FAC分类器，这是不同SU部门许多研究项目的限制因素。选定的模型是一种特殊的FACS ARIATM系统，具有来自BD生物科学的4个激光器。它满足了所有已确认的用户的需求，并且具有足够的灵活性来成功地纳入未来的用户。这将使研究人员能够将原核细胞和真核细胞分类在直径的0.2-100 m之间，高速（每秒高达70,000个细胞）和高精度（99％）。对于SU的许多正在进行的项目（包括细胞表面相互作用，药物输送和纳米技术），分子和细胞生物学（包括神经科学，细胞生理学，开发和生理学，开发和象牙间信号传导）以及涉及Algae细胞的生物学研究，这将是SU（包括细胞表面相互作用，药物输送和纳米技术）的许多正在进行的项目的重要资源。