EFRI-PSBR: Integrated design of cyanobacterial biorefineries

EFRI-PSBR：蓝藻生物精炼厂的集成设计

• 批准号: 1332404
• 负责人: Kenneth Reardon
• 金额: $ 200万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1332404&HistoricalAwards=false
• 关键词: EFRI; PSBR; Integrated; design; cyanobacterial

AbstractIntellectual Merit: Several novel systems approaches and an alternative target biofuel are brought to bear in this project to develop sustainable processes for the production of fuels and chemicals by cyanobacteria. The project has been awarded to a multidisciplinary team, consisting of Professors Kenneth F. Reardon, David S. Dandy, Thomas H. Bradley, Christie A.M. Peebles, and Graham Peers, all of Colorado State University, Fort Collins, CO. To address the goals of increasing commodity yields and increasing the sustainability of photosynthetic microbe-derived fuels, the research team will synthesize knowledge and approaches from fluid dynamics, photosynthetic physiology, proteomics, metabolic engineering, and life cycle analysis methods.In a novel approach to engineer photosynthetic efficiency, the team will model the environment of different photobioreactors and use these models to design accurate scale-down systems for laboratory cultivations, opposite to the usual practice. This will allow them to identify and target industrially relevant biology at a small scale and make accurate predictions for large-scale systems. Next, the team will quantify energy losses and stresses associated with photosynthesis, and target these for genetic improvements. This genetic research will be coupled to the metabolic engineering of cyanobacteria to shuttle carbon to a novel sesquiterpene fuel molecule, bisabolene, which offers promise as an alternative biodiesel which does not require transesterification processing, necessary in the current biodiesel production from vegetable oils. To separate the cyanobacterial cells from their cultivation liquid in an energy-efficient manner, the team will develop a novel inertial migration-based cell concentration mechanism. All of these studies on system performance will be guided by feedback from new life cycle analysis methods developed by modeling the productivity of large-scale reactors using the scale-up relationships developed in this project. The Colorado State team predicts this integrated approach to solving the major issues associated with photosynthetic microbe-based fuels will result in a rapid increase in the accuracy and speed with which the productivity of the system can be manipulated; greater yields of target molecules in industrial deployment; large, quantified increases in the overall sustainability of the process; and transferrable approaches toward the cultivation of photosynthetic microbes and the production of other biofuels or biochemicals. Broader Impacts: The proposed work, which encompasses understanding of photobioreactor process dynamics, physiological responses of cyanobacteria to photobioreactor conditions, engineering of cyanobacteria to produce biofuels and biochemicals, and life cycle analysis, will lead to photosynthetic production of advanced biofuels and biochemicals with the potential to be environmentally sustainable. The engineered strains will serve as a platform for future research on biofuel upgrading strategies and on biochemical production strategies. The project will provide interdisciplinary training for undergraduate and graduate students, as well as postdoctoral scholars, with significant efforts being made to include members of underrepresented groups. Students will gain broad experience in modeling, metabolic engineering, systems biology, microbial cell cultivation of photosynthetic organisms and life cycle analysis, placing the students at the forefront of research in biofuel production from photosynthetic organisms. The project includes opportunities for mentoring of K-12 students in research, as well as teacher training. The results of this project will be disseminated in leading peer-reviewed journals, national meetings, in new courses, and via an interactive website.

摘要智能优点：在该项目中携带了几种新型系统方法和一种替代目标生物燃料，以开发可持续的过程，以通过蓝细菌生产燃料和化学物质。该项目已被授予一个多学科团队，由肯尼斯·F·雷登（Kenneth F. Reardon），大卫·S·丹迪（David S. Peebles和Graham Peers，整个科罗拉多州立大学，Collins fort Colins co.，以解决增加商品产量的目标，并提高光合微生物衍生的燃料的可持续性，研究团队将综合知识和方法来综合流体动力学，光合物理学，光合作用的方法，蛋白质组学，代谢工程和生命周期分析方法。在一种新型的工程方法光合作用效率的方法中，该团队将模拟不同光生反应器的环境，并使用这些模型设计准确的规模降低系统以进行实验室培养，与通常的实践相反。这将使他们能够小规模识别和靶向与工业相关的生物学，并对大规模系统做出准确的预测。接下来，团队将量化与光合作用相关的能量损失和应力，并将其定为遗传改善。这项遗传研究将与蓝细菌的代谢工程相结合，以将碳穿梭到新型的倍半萜烯燃料分子Bisabolene，该分子Bisabolene提供了有望作为替代生物柴油的希望，该柴油不需要跨酯化处理，在当前从蔬菜油中生产的生物柴油在当前的生物柴油中必不可少。为了以节能方式将蓝细菌细胞与其培养液体分开，该团队将开发出一种新型的基于惯性迁移的细胞浓度机制。所有这些关于系统性能的研究将以新的生命周期分析方法的反馈来指导，该方法通过使用本项目中开发的扩大关系来对大规模反应堆的生产率进行建模。科罗拉多州小组预测，这种综合方法可以解决与基于光合微生物的燃料相关的主要问题，这将导致操纵系统生产率的准确性和速度迅速提高；目标分子在工业部署中的产量更高；该过程的总体可持续性大大增加；以及用于培养光合微生物以及其他生物燃料或生化物质的可转让方法。更广泛的影响：拟议的工作涵盖了对光生反应器过程动态的了解，蓝细菌对光生反应条件的生理反应，蓝细菌的工程性以产生生物燃料和生物化学，以及生命周期分析，将导致与先进的生物策略的光合作用生产。在环境上可持续。 工程菌株将作为生物燃料升级策略和生化生产策略的未来研究的平台。 该项目将为本科生和研究生以及博士后学者提供跨学科的培训，并为包括代表性不足的群体的成员所做的重大努力。 学生将在建模，代谢工程，系统生物学，光合生物的微生物细胞培养和生命周期分析方面获得广泛的经验，使学生处于来自光合生物的生物燃料生产研究的最前沿。 该项目包括指导K-12学生研究和教师培训的机会。 该项目的结果将在领先的同行评审期刊，国家会议，新课程中以及通过交互式网站中传播。