Interplay of Transcriptional, Translational Regulatory Mechanisms and Kinetics of an Environmental Response

转录、翻译调节机制和环境反应动力学的相互作用

• 批准号: 1330912
• 负责人: Nitin Baliga
• 金额: $ 120万
• 依托单位: Institute for Systems Biology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330912&HistoricalAwards=false
• 关键词: Interplay; Transcriptional; Translational; Regulatory; Mechanisms

INTELLECTUAL MERIT: Environmental factors change with reproducible characteristics. While changes in oxygen can happen within seconds to minutes, temperature changes occur at a much slower pace over hours to days. Regulatory networks in an organism are tuned through evolution to elicit a response that matches the characteristics of a given environmental factor (e.g. a rapid response to fast changing factors). The overarching hypothesis of this project is that the relative use of regulation at different stages of information processing (transcriptional, post-transcriptional, translational and post-translational) is dictated by the necessary characteristics of an environmental response. In order to test this hypothesis under varying environmental conditions, changes in synthesis and degradation rates will be measured for all transcripts and proteins in strains of Halobacterium salinarum. Building in this proof-of-concept, the research project will systematically investigate interplay across different levels of regulation in the context of specific environmental responses.BROADER IMPACTS:Resources: This research will generate fundamental principles of integration across different levels of regulation, and also generate innovative computational and experimental methodology that will be published as well-documented, open-source data and software. Education: Rapid advances in interdisciplinary science are widening the gap in the way biology is taught and how it is practiced. An award winning high school (HS) education program in the lab is bridging this divide through inquiry-based teaching practices at the grassroots. This ten-year-old program is centered on summer internships for students of disadvantaged backgrounds who are encouraged to apply through numerous partnerships including programs for inner city youth and a UW-based program for advancement of women in science. Educational kits developed through this program have been aligned with state standards, iteratively improved through classroom pilots, and disseminated through professional teacher development to 14 states across the US. In addition to training postdoctoral fellows in systems biology, this project will also develop inquiry-driven, standards-based, HS educational materials. Developed together with local science educators, this novel curriculum module has been requested by teachers and will help students internalize the concepts and methods for using systems biology to understand cellular dynamics in the context of environmental change. These curricula will be aligned with the new Next Generation Science Standards and regularly assess its impact on student learning and career choices through a rigorous evaluation program.

智力优点：环境因素随可重复的特征而变化。尽管氧气的变化可能会在几秒钟到几分钟内发生，但温度变化在数小时至几天内的速度速度较慢。通过进化来调整生物体中的调节网络，以引起与给定环境因素的特征相匹配的响应（例如，对快速变化的因素的快速响应）。该项目的总体假设是，在信息处理的不同阶段（转录，转录后，翻译和翻译后）的调节相对使用取决于环境响应的必要特征。为了在不同的环境条件下检验这一假设，将测量盐酸盐菌株中所有转录本和蛋白质的合成和降解速率的变化。 在概念验证验证的基础上，该研究项目将在特定环境响应的背景下系统地研究不同级别的调节的相互作用。BRODER的影响：资源：资源：这项研究将产生跨不同级别调节的集成基本原理，并生成创新的计算和实验方法，这些方法将发表，并将备受纪录的内容以及开放式的数据和软件数据发表。 教育：跨学科科学的快速进步正在扩大生物学教学方式以及如何实践的差距。实验室中屡获殊荣的高中教育计划正在通过基于基层的基于询问的教学实践来弥合这种鸿沟。这个十岁的计划集中在暑期实习面向弱势背景的学生的暑期实习，他们被鼓励通过众多合作伙伴关系申请，包括内城青年计划和基于UW的科学发展计划。通过该计划开发的教育套件已与州标准一致，通过课堂飞行员进行了迭代的改进，并通过专业教师发展传播给了美国的14个州。除了培训系统生物学的博士后研究员外，该项目还将开发以询问为基础的基于标准的HS教育材料。教师已要求与当地科学教育者一起开发，这是该新颖的课程模块，将帮助学生将使用系统生物学的概念和方法内部化，以了解环境变化的背景下的细胞动态。这些课程将与新的下一代科学标准保持一致，并通过严格的评估计划定期评估其对学生学习和职业选择的影响。