NSF East Asia and Pacific Summer Institute (EAPSI) for FY 2013 in China

2013 财年 NSF 东亚及太平洋暑期学院 (EAPSI) 在中国

• 批准号: 1316983
• 负责人: Nicholas Nuar
• 金额: $ 0.53万
• 依托单位: Nuar Nicholas C
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1316983&HistoricalAwards=false
• 关键词: NSF; East; Asia; Pacific; Summer; NSF; East; Asia; Pacific; Summer

This action funds Nicholas Nuar of The University of Pittsburgh Department of Bioengineering to conduct a research project in Biology during the summer of 2013 at Peking University in Beijing, China. The project title is "Biosensor Development Using Membrane Protein Receptors." The host scientist is Dr. Can Xie.Membrane receptors are proteins embedded in the cellular membrane that mediate the communication between the cell and its environment. It is therefore intuitive to use membrane receptors for biosensor development for technological applications in a biomimetic approach. The principle is general so that such a technology platform can be extended to potentially be able to detect and assay thousands of biologically relevant compounds. This project extends the system to a plant membrane receptor, the ethylene receptor, studied by Prof. Can Xie in China. Ethylene (C2H4) is an important plant growth substance that is used industrially to induce ripening in stored plant products. Its receptor is not homologous to the insulin receptor and its transmembrane structure is quite different, and kinase activity is either histidine or serine/threonine specific. This study investigates a plant ethylene receptor, NTHK1. Unique among identified plant hormone receptors, the ethylene receptors are transmembrane receptors just like hormone receptors, such as EGFR in animals. This activity studies the expression, purification, and assay setup necessary to continue investigating the protein in collaboration with Dr. Xie. The ultimate goal of the Fellow's PhD project is to harness understanding of these highly tuned receptor kinase systems to make an extensible platform for novel biosensing capabilities. This project will have an impact on the speed, accuracy, and number of readily analyzable chemical species. Broader impacts of an EAPSI fellowship include providing the Fellow a first-hand research experience outside the U.S.; an introduction to the science, science policy, and scientific infrastructure of the respective location; and an orientation to the society, culture and language. These activities meet the NSF goal to educate for international collaborations early in the career of its scientists, engineers, and educators, thus ensuring a globally aware U.S. scientific workforce. Furthermore, the Fellow will strengthen a collaboration between the Klein-Seetharaman lab and the Xie laboratories. High impact, low cost solutions to laboratory instrumentation are ultimate downstream aims from the program of research.

这项行动为匹兹堡大学生物工程系的尼古拉斯·努尔（Nicholas Nuar）提供了资金，以在2013年夏季在中国北京的北京大学进行生物学研究项目。 该项目的标题是“使用膜蛋白受体的生物传感器开发”。宿主科学家是Can Xie。膜受体是嵌合在细胞膜中的蛋白质，可介导细胞与其环境之间的通信。因此，将膜受体使用生物传感器开发进行技术应用是直观的。该原则是一般的，因此可以扩展这样的技术平台，以便能够检测和测定数千种与生物学相关的化合物。该项目将系统扩展到植物膜受体，即乙烯受体，由Can Xie教授在中国研究。乙烯（C2H4）是一种重要的植物生长物质，在工业上用于诱导储存的植物产品成熟。它的受体与胰岛素受体并非同源，其跨膜结构截然不同，激酶活性是组氨酸或丝氨酸/苏氨酸的特异性。这项研究研究了植物乙烯受体NTHK1。在已鉴定的植物激素受体中，乙烯受体独有的是跨膜受体，就像激素受体一样，例如动物中的EGFR。该活动研究了与XIE博士合作继续研究蛋白质所需的表达，纯化和测定设置。同伴博士项目的最终目标是利用对这些高度调整的受体激酶系统的理解，以为新颖的生物传感能力建立可扩展的平台。该项目将对易于分析的化学物种的速度，准确性和数量产生影响。 EAPSI奖学金的更广泛的影响包括为这位美国境外的第一手研究经验提供；各自位置的科学，科学政策和科学基础设施的简介；以及社会，文化和语言的方向。 这些活动符合NSF的目标，以在其科学家，工程师和教育工作者的职业生涯的早期对国际合作进行教育，从而确保全球意识的美国科学劳动力。 此外，这位研究员将加强克莱因·塞萨拉曼实验室与Xie实验室之间的合作。高影响力，低成本的实验室仪器解决方案是研究计划的最终下游目标。