AgriSense: Multichannel disposable sensors for animal health disease diagnostics

AgriSense：用于动物健康疾病诊断的多通道一次性传感器

• 批准号: 1264705
• 负责人: Eric Vogel
• 金额: $ 35.84万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-15 至 2017-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1264705&HistoricalAwards=false
• 关键词: AgriSense; Multichannel; disposable; sensors; animal

1264705(Vogel). Rapid and sensitive detection of bovine respiratory disease (BRD)-associated viral infections in live animals is recognized as key to minimizing the impact of this disease. Currently, the most common method of serological diagnosis is fluorescently labeled ELISAs. ELISA-based testing is limited as it typically relies on the detection of a single viral antibody subtype within an individual test sample per ELISA plate, the testing is relatively slow (up to 1 week) and expensive (~$5 per test). A low-cost multiplexed platform capable of detecting several antibody markers related to BRD infections in the same test assay would offer significant economic benefits for routine serological testing. Additionally, rapid on-farm ?cowside? multiplexed diagnostic capabilities would be a major step forward towards more efficient and effective management, control and/or eradication of BRD-associated pathogens. At present there is no electronic field based test for disease diagnostics available commercially for the animal health sector, let alone for BRD. Our team will address these challenges by building inexpensive electrochemical sensors for detection of biomarkers associated with BRD infection.

1264705（沃格尔）。快速、灵敏地检测活体动物中与牛呼吸道疾病 (BRD) 相关的病毒感染被认为是最大限度减少该疾病影响的关键。目前，最常用的血清学诊断方法是荧光标记的ELISA。基于 ELISA 的测试是有限的，因为它通常依赖于每个 ELISA 板的单个测试样本中单个病毒抗体亚型的检测，测试相对较慢（长达 1 周）且昂贵（每次测试约 5 美元）。能够在同一测试测定中检测与 BRD 感染相关的多种抗体标记物的低成本多重平台将为常规血清学测试提供显着的经济效益。此外，快速农场“牛边”多重诊断能力将是朝着更高效和有效地管理、控制和/或根除 BRD 相关病原体迈出的重要一步。目前，动物健康部门还没有商业化的基于电子现场的疾病诊断测试，更不用说 BRD 了。 我们的团队将通过构建廉价的电化学传感器来检测与 BRD 感染相关的生物标志物来应对这些挑战。