Collaborative Research: Development of Preclinical X-Ray Phase Contrast Tomography for 3D Imaging of Engineered Tissues

合作研究：开发用于工程组织 3D 成像的临床前 X 射线相衬断层扫描

• 批准号: 1263988
• 负责人: Mark Anastasio
• 金额: $ 27.5万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-10-01 至 2017-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1263988&HistoricalAwards=false
• 关键词: Collaborative; Research; Development; Preclinical; Ray

1263988/1263994 Anastasio/BreyThe field X-ray imaging is being revolutionized by the development of techniques based on phase-contrast (PC), which have shown great promise for soft tissue imaging. The principal advantage is that X-ray PC imaging methods are sensitive to alternative physical properties of tissues, and can differentiate tissues that have very similar or even identical X-ray absorption properties. The broad objective of this research program is to develop and comprehensively evaluate X-ray PC imaging and tomography for use in the evaluation and monitoring of engineered tissues. The specific research objects of the project are to: (1) Investigate non-traditional mechanisms of X-ray contrast produced by model-engineered tissues and determine conditions for optimal imaging of polymer and tissue structures ex vivo and in culture conditions; (2) Optimize grating-based PC imager designs and image reconstruction algorithms for volumetric imaging of engineered tissues; and (3) Evaluate a benchtop PC imager for 3D in vivo evaluation of engineered tissues in small animal models. The intellectual merit of the proposed research arises from the development and refinement of hardware designs and image formation algorithms for a new X-ray imaging modality and their application to the important problem of characterizing and monitoring engineered tissues. Novel X-ray contrast mechanisms that can effectively characterize engineered tissues will be systematically investigated. Model polymer scaffolds and engineered tissues will be used in the evaluation and optimization of these imaging systems in order to ensure broad relevance to many areas of tissue engineering, regenerative medicine and biomaterials. To accomplish this research, a combination of physics and fundamental principles of biomedical engineering and imaging science are employed by our collaborative research team. There are several broad impacts of the project that will yield important benefits to both biomedical science and society. The successful completion of this project will establish X-ray PC imaging as a highly effective tool for characterizing the 3D structure of engineered tissues and will have a transformative impact on the field of tissue engineering. The acceleration of tissue engineering research will have a significant impact on human health by leading to new therapeutic approaches for the treatment of defects resulting from disease, trauma or congenital defects. The integration of this research with the proposed educational activities will help attract students to the increasingly important fields of biomedical engineering, biomedical imaging and tissue engineering and enhance greatly their educational opportunities.

1263988/1263994 Anastasio/Breythe Field X射线成像正在通过基于相对比（PC）的技术的发展进行了革命，这些技术对软组织成像显示了很大的希望。主要优点是X射线PC成像方法对组织的替代物理特性敏感，并且可以区分具有非常相似甚至相同X射线吸收特性的组织。该研究计划的广泛目的是开发和全面评估X射线PC成像和断层扫描，用于评估和监测工程组织。该项目的特定研究对象是：（1）研究由模型工程组织产生的X射线对比度的非传统机制，并确定在体外和培养条件下聚合物和组织结构的最佳成像的条件； （2）优化基于光栅的PC成像器设计和图像重建算法，用于工程组织的体积成像； （3）评估小动物模型中工程组织的3D评估台式PC成像仪。拟议研究的智力优点源于用于新的X射线成像模式的硬件设计和图像形成算法的开发和完善，以及它们在表征和监测工程组织的重要问题上的应用。可以系统地研究可以有效表征工程组织的新型X射线对比机制。模型聚合物支架和工程组织将用于评估和优化这些成像系统，以确保与组织工程，再生医学和生物材料的许多领域的广泛相关性。为了完成这项研究，我们的协作研究团队采用了生物医学工程和成像科学的物理学和基本原理的结合。该项目有一些广泛的影响，将为生物医学科学和社会带来重要的好处。该项目的成功完成将建立X射线PC成像作为表征工程组织的3D结构的高效工具，并将对组织工程领域产生变革性的影响。组织工程研究的加速性将对人类健康产生重大影响，从而导致新的治疗方法治疗疾病，创伤或先天性缺陷引起的缺陷。这项研究与拟议的教育活动的整合将有助于吸引学生进入越来越重要的生物医学工程，生物医学成像和组织工程领域，并增强了他们的教育机会。