Division Plane Orientation in Plant Cells

植物细胞的分割面方向

• 批准号: 1244202
• 负责人: Carolyn Rasmussen
• 金额: $ 52.1万
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244202&HistoricalAwards=false
• 关键词: Division; Plane; Orientation; Plant; Cells

Plants have two methods for establishing a body plan: division and expansion. Therefore, understanding cell division, particularly the coordination between cell division and differentiation mediated by correct orientation of the division plane, is crucial to understanding plant development. Moreover, division plane orientation is vital for all eukaryotic cells, and plants serve as useful model organisms for studying this process. Little is known about the factors that control proper orientation of the division plane in plants, however, recent data suggests that sequential modification of the plant division site is essential for the final proper orientation of cell division. This research identifies the proteins responsible for controlling division plane orientation in both Arabidopsis thaliana and maize. The experimental approaches include live cell imaging, biochemistry, and reverse and forward genetics. The expected outcome of this research is identification of a plant division site interactome placed within the temporal context of the cell cycle and within the interacting network of other proteins. This outcome will have important positive impact because elucidating the process of plant division plane orientation will provide new tools and resources for modifying plants through control of cell division planes which may lead to next generation improvements in crop plants.Broader ImpactsThis project, focusing on fundamental understanding of cell division dynamics in plants, has the potential for long-term application to crop plants and will also provide useful training and educational opportunities for students in the broader Wyoming community. The project integrates research with training and education by providing student workshops for undergraduates and community college transfer students at University of Wyoming that use developmental genetics and imaging resources described in this project as training tools. Students will initiate research on selected mutants as part of a standard laboratory course in the Molecular Biology curriculum, and then will have the opportunity to apply for competitive student funding from the NSF Wyoming EPSCoR program for summer research. Community college students will be offered two-day workshops in A. thaliana imaging and genetics to supplement already established activities designed to introduce community college students to opportunities as undergrads at University of Wyoming. The project aims to broaden participation of under-represented groups through development of a research program headed by a female principal investigator with the goal of attracting new scientists into careers in high-priority areas of national need (agriculture and plant sciences), making use of internally and externally funded programs at University of Wyoming. Specifically the investigator will host 7th and 8th grade girls at an Annual Women and Girls in Science Day. Students will be encouraged to communicate with the investigator via Facebook as they enter the high school science curriculum. In addition to enhancing scientific and technological understanding by dissemination of project results through peer-reviewed publications and presentations at national conferences, the project will benefit society by advancing knowledge of the biology of plant division site orientation. In the long-term, the outcomes of the research will be translated to and complemented by new findings in crop plants.

植物有两种建立身体计划的方法：分裂和扩展。因此，了解细胞分裂，尤其是细胞分裂之间的协调和分化平面的介导的分化，对于理解植物发育至关重要。此外，除法平面方向对于所有真核细胞至关重要，并且植物是研究此过程的有用模型生物。但是，对控制植物中分裂平面的正确取向的因素知之甚少，但是，最近的数据表明，对植物分裂位点的顺序修饰对于最终的细胞分裂正确定向至关重要。这项研究确定了负责控制拟南芥和玉米的分裂平面取向的蛋白质。实验方法包括活细胞成像，生物化学以及反向和正向遗传学。这项研究的预期结果是识别植物分裂位点相互作用组，放置在细胞周期的时间环境以及其他蛋白质的相互作用网络中。该结果将产生重要的积极影响，因为阐明植物分裂平面的过程将通过控制细胞分裂平面来修饰植物的新工具和资源，这可能会导致作物植物的下一代改善。植物中的细胞分裂动态具有长期应用作物植物的潜力，还将为更广泛的怀俄明州社区的学生提供有用的培训和教育机会。该项目通过为怀俄明大学的大学生和社区大学转学学生提供学生研讨会，将研究与培训和教育相结合，这些讲习班使用该项目中描述的发展遗传学和成像资源作为培训工具。作为分子生物学课程的标准实验室课程的一部分，学生将开始研究选定的突变体，然后有机会向NSF Wyoming Epscor计划申请有竞争力的学生资金，以进行夏季研究。社区大学的学生将在A. Thaliana成像和遗传学上提供为期两天的研讨会，以补充已经建立的活动，旨在向社区大学生介绍怀俄明大学的本科生的机会。该项目旨在通过制定由女性首席研究人员领导的研究计划来扩大代表性不足的群体的参与，目的是吸引新科学家从事国家需求的高优先级领域（农业和植物科学）的职业，并利用怀俄明大学的内部和外部资助课程。特别是，调查员将在科学日的年度妇女和女孩中接待7年级和8年级的女孩。将鼓励学生在进入高中科学课程时通过Facebook与调查员进行沟通。除了通过在国家会议上通过同行评审的出版物和演讲来扩展项目结果来增强科学和技术理解外，该项目还将通过促进对植物分区现场培养的生物学知识来使社会受益。从长远来看，这项研究的结果将被农作物植物中的新发现转化并补充。