Structure Determination of Photosynthetic Organelles

光合细胞器的结构测定

• 批准号: 1240590
• 负责人: Cheryl Kerfeld
• 金额: $ 29.99万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1240590&HistoricalAwards=false
• 关键词: Structure; Determination; Photosynthetic; Organelles

Intellectual Merit:This project takes advantage of the latest development in X-ray Free Electron Lasers (XFELs) at the Linac Coherent Lisht Source (LCLS) to structurally characterize intact photosynthetic organelles, chlorosomes and carboxysomes. The research undertaking embodies high-risk, high reward research since every experiment undertaken at the LCLS is at the frontier of imaging biology which continues to be under development. Chlorosomes and carboxysomes have not yet been subjected to these methods. Both types of complexes provide unprecedented test cases for XFELs diffraction in that they can both be treated as single particle experiments but with crystalline components. Particles of this size and complexity have not yet been characterized. This is a high risk project in that it is difficult to anticipate the characteristics of the resulting data from these "diffract and destroy" experiments. Methods for processing and analyzing these data will be developed. The results of the project will lay the foundation for visualization of functioning macromolecular organelles. Broader Impact:The broader impact of the project is associated with the training of young scientists at arguably the most exciting intersection of math, physics and biology in a large (9 groups) international collaboration. The pioneering methods developed will be useful for advancing the community of researchers gaining access to LCLS beam-time.This project is jointly funded by the Biomolecular Dynamics Structure and Function Cluster and the Networks and Regulation Cluster.

知识分子优点：该项目利用了Linac Cooherent Lisht源（LCLS）的最新开发X射线无电子激光器（XFELS），从而在结构上表征了完整的光合器细胞器，叶绿体和羧化合物。这项研究实现了高风险，高奖励研究，因为在LCLS进行的每个实验都处于成像生物学的边界，该研究继续开发。氯化体和羧化体尚未受到这些方法的影响。两种类型的复合物都为XFELS衍射提供了前所未有的测试用例，因为它们都可以视为单个粒子实验，但用晶体组件将其视为。这种大小和复杂性的颗粒尚未表征。这是一个高风险项目，因为很难从这些“衍射和破坏”实验中预测所得数据的特征。将开发处理和分析这些数据的方法。该项目的结果将为可视化大分子细胞器的可视化奠定基础。更广泛的影响：该项目的更广泛影响与在大型（9组）国际合作中最令人兴奋的数学，物理和生物学交叉点可以说是对年轻科学家的培训。开发的开创性方法将有助于推进研究人员获得LCLS Beam时间的访问。该项目由生物分子动力学结构和功能群集以及网络和调节群集共同资助。