COLLABORATIVE RESEARCH: Virus removal in membrane bioreactors: Role of virus aggregation and adhesion

合作研究：膜生物反应器中的病毒去除：病毒聚集和粘附的作用

• 批准号: 1236672
• 负责人: Thanh Nguyen
• 金额: $ 19.06万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1236672&HistoricalAwards=false
• 关键词: COLLABORATIVE; RESEARCH; Virus; removal; membrane

1236672/1236393Nguyen/Tarabara Aerobic membrane bioreactors (MBRs) have become a state-of-the-art technology for municipal and industrial wastewater treatment. Compared to traditional activated sludge reactors, advantages of MBR include smaller footprint and better effluent quality. MBRs have become a particularly attractive treatment choice for water reuse. In reuse applications or when the effluent is released to sensitive water bodies such as recreational water, it is especially important that viral pathogens are removed so that public health is not threatened. Comprehensive studies of virus fate in MBRs are currently lacking yet required for wider application of MBR technology, including decentralized water reuse with variable influent quality. The PIs will systematically investigate the role of virus adhesion and aggregation on virus removal in MBRs. The central hypothesis is that the extent of virus adhesion to extracellular polymeric substances (EPS) and soluble microbial products (SMP) counter-correlates with virus passage through the membrane. The experimental plan consists of the following tasks: (1) conduct bench-scale aerobic MBR studies with flat sheet and hollow fiber membranes of varied pore size; in a subset of tests operating conditions will mimic those used in the CH2M Hill full-scale MBR facility in Traverse City, MI, (2) characterize SMP, EPS, and membrane biofilm harvested from the bench-scale MBR system and the full-scale MBR plant, (3) quantify virus aggregation and adhesion to fouled membranes using SMP and EPS characterized in Task 2, (4) correlate adhesion and aggregation with the removal of virus by fouled membranes at different stages of biofilm development, and (5) evaluate virus removal in MBRs using cell culture-dependent methods and quantitative PCR. Adenovirus is selected as target virus for this study. The findings will have the potential for transforming MBR design criteria particularly as they pertain to membrane selection. While MBR-based treatment relies on the membrane to ensure an appropriate separation barrier, virus removal has not been a criterion in designing MBRs. As our technological, economic and social attitudes and approaches toward water shift from the unidirectional resource-to-waste approach toward the concept of sustainable reuse, stricter control over microbiological quality of treated used water becomes a question of when rather than if. With this in mind, we see the practical end of the research we propose in informing the decision-making process reqiered for the development of water reuse policies and regulations of the near future. Successful completion of this project will provide scientific basis for re-conceptualizing MBR design and operation to accommodate virus removal as one of design criteria and treatment goals. The project brings together a diverse team of PIs with a complimentary set of skills including interfacial science, membrane processes, and environmental microbiology and an industrial partner representing a full-scale MBR facility. A group of graduate and undergraduate students co-advised by all PIs will be provided a range of learning opportunities in an environment that combines academic studies, laboratory research in collaboration with the industrial sector, and international work. We will organize annual mini-symposia - at UIUC (year 1), MSU (year 2), and at the Traverse City MBR facility (year 3) where project participants will present their findings, discuss future research and receive feedback from the industrial partner.

1236672/1236393NGUYEN/TARABARA有氧膜生物反应器（MBRS）已成为市政和工业废水处理的最先进技术。与传统的活性污泥反应堆相比，MBR的优势包括较小的足迹和更好的废水质量。 MBR已成为重用水的特别有吸引力的治疗方法。在重复使用应用或释放到诸如娱乐水之类的敏感水体中，去除病毒病原体以使公共卫生不会受到威胁，这一点尤其重要。目前缺乏对MBR的病毒命运的全面研究，但仍需要更广泛的MBR技术应用，包括分散的水再利用，其影响力可变。 PIS将系统地研究病毒粘附和聚集在MBR中的病毒去除的作用。中心假设是，病毒粘附于细胞外聚合物（EPS）和可溶性微生物产物（SMP）与病毒穿过膜的相交的程度。实验计划由以下任务组成：（1）用孔径变化的平板和空心纤维膜进行台式有氧运动MBR研究； in a subset of tests operating conditions will mimic those used in the CH2M Hill full-scale MBR facility in Traverse City, MI, (2) characterize SMP, EPS, and membrane biofilm harvested from the bench-scale MBR system and the full-scale MBR plant, (3) quantify virus aggregation and adhesion to fouled membranes using SMP and EPS characterized in Task 2, (4)在生物膜发育的不同阶段通过污染膜去除病毒的粘附和聚集，以及（5）使用细胞培养依赖性方法和定量PCR评估MBR中的病毒去除。 腺病毒被选为这项研究的靶向病毒。这些发现将有可能改变MBR设计标准，尤其是与膜选择有关。尽管基于MBR的治疗依赖于膜来确保适当的分离屏障，但切除病毒并不是设计MBR的标准。随着我们的技术，经济和社会态度以及水从单向资源到垃圾的方法转移到可持续再利用概念的转变，对处理过的用水的微生物学质量的更严格控制成为一个问题，即何时而不是何时。考虑到这一点，我们看到了我们提出的研究的实际终结，以告知决策过程，以制定不久的将来的水再利用政策和法规。 该项目的成功完成将为重新概念化MBR设计和操作提供科学基础，以适应病毒去除，作为设计标准和治疗目标之一。该项目汇集了一个多元化的PI团队，并提供了一套免费的技能，包括界面科学，膜过程和环境微生物学，以及代表全面MBR设施的工业合作伙伴。在将所有PI共同努力的一组研究生和本科生将在结合学术研究，与工业领域合作的实验室研究以及国际工作的环境中提供一系列学习机会。我们将在UIUC（第1年），MSU（2年级）和Traverse City MBR设施（第3年）组织年度迷你群岛 - 项目参与者将提出他们的发现，讨论未来的研究并接收工业合作伙伴的反馈。