A Multi-Spectroscopic Investigation of Protein Structure in Biosilica Composites

生物二氧化硅复合材料中蛋白质结构的多光谱研究

• 批准号: 1219509
• 负责人: Gary Drobny
• 金额: $ 35万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-15 至 2016-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1219509&HistoricalAwards=false
• 关键词: Multi; Spectroscopic; Investigation; Protein; Structure

With this award, the Chemistry of Life Processes Program is funding a collaboration between the solid state NMR (ssNMR) group of Gary Drobny at the University of Washington, Seattle, USA, and the optical spectroscopy group of Tobias Weidner at the Max Planck Institute for Polymer Research, Mainz, Germany. The NSF will be funding the U.S. team and the Deutsche Forshungsgemeinshaft will be funding the German team. This collaboration is aimed at elucidating to high resolution, the structures of proteins and peptides in biosilica composites. Not only will this research provide an atomic level structural picture of how proteins structure when in contact with silica, but the information will be helpful in understanding how biogenic silica is modeled into elaborate macroscopic structures under relatively mild conditions compared to the extreme conditions (high temperatures, pressures etc.) that are involved in the production and manipulation of anthropogenic silica.There are potential long term goal implications of these collaborative studies toward understanding the nature of an important problem in chemical biology; namely peptide-silica interactions and the roles played by peptide secondary, tertiary, and quaternary structure as well as amino acid modifications in controlling the morphology of biosilica composites. These approaches will develop further a set of spectroscopic techniques for structurally characterizing proteins as they exist in other types of biomineral composites. The interdisciplinary and international nature of the collaboration is expected to be beneficial for both the U.S. scientists funded by the NSF and the German scientists funded by the Deutsche Forschungsgemeinshaft.

通过该奖项，《生命过程》计划的化学计划正在为美国西部西雅图大学华盛顿大学的Gary Drobny组的固态NMR（SSNMR）小组与Max Planck Planck Planck Polymer研究所Max Planck Polymer Research of Tobias Weidner组成的合作。 NSF将为美国团队提供资金，而德意志福斯格梅沙夫（Deutsche forshungsgemeinshaft）将为德国团队提供资金。 该合作旨在阐明生物硅化物复合材料中蛋白质和肽的结构。这项研究不仅会提供与二氧化硅接触时蛋白质结构如何结构的结构图，而且这些信息将有助于理解在相对温和的条件下如何将生物基因二氧化硅建立在相对温和的条件下，与极端条件相比（高温，压迫等）的生产和涉及这些偏见的潜在的远离硅的影响，这些偏见与涉及的较重要的启用了这些偏见。化学生物学；即肽 - 二氧化硅相互作用以及肽二级，第三纪和第四纪结构以及氨基酸修饰在控制生物硅利亚复合材料的形态方面所发挥的作用。这些方法将进一步开发一组光谱技术，用于在其他类型的生物矿物复合材料中存在结构表征蛋白质。 该合作的跨学科和国际性质预计将对由NSF资助的美国科学家和德国科学家资助的美国科学家都有益。