CAREER: A phylogenetic and functional understanding of microbial sulfur cycling in oxygen minimum zones
职业:对最低氧区微生物硫循环的系统发育和功能理解
基本信息
- 批准号:1151698
- 负责人:
- 金额:$ 121.09万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-02-15 至 2020-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Oxygen concentration significantly impacts the community structure and function of marine ecosystems. In waters with low oxygen, including the major marine oxygen minimum zones (OMZs), biological diversity is dominated by a complex community of microorganisms whose anaerobic metabolisms mediate key steps in global nitrogen and carbon cycles. Surprisingly, new evidence indicates that OMZs also support diverse microorganisms capable of utilizing inorganic sulfur compounds for energy metabolism. This assemblage appears to include both sulfur-oxidizing autotrophs and sulfate-reducing heterotrophs, suggesting an active sulfur cycle with potentially substantial roles in organic carbon input and mineralization, as well as critical links to the OMZ nitrogen cycle. Our knowledge of the microorganisms driving OMZ sulfur cycling is based largely on the metagenome of a single bacterial lineage (SUP05) and on surveys of diagnostic marker genes, which have thus far targeted only a subset of the diverse low-oxygen regions in the global ocean. The metabolic diversity, activity, and biogeographic distribution of sulfur-metabolizing microorganisms in the OMZ water column remain largely unexplored. This project uses an integrated molecular and experimental approach to critically examine the physiological and phylogenetic basis of microbial sulfur cycling in oxygen minimum zones. Combining targeted metagenomics with gene expression profiling, microcosm sulfur-addition experiments, and enrichment culturing, the PI will characterize sulfur-metabolizing microorganisms in two oceanographically and ecologically distinct low-oxygen regions: the Eastern Tropical North Pacific (ETNP) OMZ off Mexico, which represents the largest permanent OMZ in the world, and the seasonally hypoxic "dead zone" in the Gulf of Mexico (GOM). Specifically, they will test the hypotheses that sulfur- oxidizing and -reducing bacterioplankton 1) are abundant and transcriptionally active in the ETNP OMZ, 2) are minor components of the hypoxic GOM, but increase in activity and abundance when oxygen decreases and sulfide increases, and 3) exhibit biogeographic variation in functional gene content and phylogenetic diversity over vertical profiles, among OMZs, and in response to environmental gradients. OMZs are predicted to expand in response to future climate change, making it imperative to holistically understand the biology of low-oxygen regions. This project will establish a comprehensive framework for studying the genomics and physiology of an ecologically important, but poorly characterized, functional group(s) of marine bacterioplankton in OMZs. Results will be analyzed relative to existing metagenomic data from the permanent Eastern Tropical South Pacific (ETSP) OMZ, and a second seasonal OMZ (Saanich Inlet), thereby establishing a comparative basis for describing the ecological distribution of pelagic sulfur-metabolizing microorganisms and their relative role in OMZ community metabolism.Broader ImpactsMarine science research will be used as a platform for enhancing science education across multiple academic levels. A Summer Workshop in Marine Science (SWIMS) will be developed in collaboration with K-12 educators and teacher-development experts at Georgia Tech. The 5-day SWIMS program, which includes 2 days at the Skidaway Institute of Oceanography, will engage graduate students and Georgia Tech researchers in training local teachers to merge key topics in marine science with new national standards in middle school Earth Science education. In addition, through a partnership with Morehouse and Spelman Colleges, this project funds summer internships to enhance representation of minority students in undergraduate marine science and bioinformatics research. A PI-graduate student working group is to be established at Georgia Tech to develop and implement new guidelines for training in the cross-disciplinary field of marine genomics. A new course will be created at Georgia Tech, Writing for Scientists, to enhance the professional development of graduate students. It will use structured, peer-driven exercises to equip students with the critical writing and speaking skills necessary for success in science - such a course is critically lacking at Georgia Tech. Through the activities outlined above, this CAREER project will not only establish a research group dedicated to characterizing an ecologically significant, but cryptic, component of the marine microbial ecosystem, but will also develop a strong foundation through which the PI can use the results of his work to train and motivate future generations of marine scientists.
氧气浓度显着影响海洋生态系统的社区结构和功能。在氧气低的水域(包括主要的海氧最小区域(OMZ))中,生物多样性由复杂的微生物群落主导,其厌氧代谢介导了全球氮和碳循环中的关键步骤。令人惊讶的是,新的证据表明,OMZ还支持能够利用无机硫化合物来代谢的多种微生物。该组合似乎包括硫酸氧化自身营养和硫酸盐还原的异嗜性,这表明有效的硫循环在有机碳输入和矿化中具有潜在的重要作用,以及与OMZ氮循环的关键联系。我们对驱动OMZ硫循环的微生物的了解主要基于单个细菌谱系(SUP05)的元基因组(SUP05)和诊断标记基因的调查,这些标记基因迄今仅针对全球海洋中多样的低氧区域的子集。 OMZ水柱中硫代谢微生物的代谢多样性,活性和生物地理分布在很大程度上尚未探索。该项目使用综合分子和实验方法来批判性地检查氧最小区域中微生物硫循环的生理和系统发育基础。 PI将靶向的宏基因组与基因表达分析,微观硫化实验和富集培养相结合,PI将在两个海洋和生态上不同的低氧区域中硫酸代谢微生物的特征:东部热带北部太平洋(ETNP)Off MEXENT和MEDENTS OMESICT,这是一个始终的媒体,这是一个季节性的,这是一个范围的,这是一个范围,这是一个范围的范围,这是一个范围的范围。墨西哥湾(GOM)的“死区”。 Specifically, they will test the hypotheses that sulfur- oxidizing and -reducing bacterioplankton 1) are abundant and transcriptionally active in the ETNP OMZ, 2) are minor components of the hypoxic GOM, but increase in activity and abundance when oxygen decreases and sulfide increases, and 3) exhibit biogeographic variation in functional gene content and phylogenetic diversity over vertical profiles, among OMZ,并响应环境梯度。预计OMZ会响应未来的气候变化而扩展,因此必须从整体上了解低氧区域的生物学。该项目将建立一个综合框架,用于研究OMZ中海洋菌孢兰克顿的生态重要但特征性的功能群的基因组学和生理学。将分析结果相对于现有东部热带东部太平洋(ETSP)OMZ的现有宏基因组数据,以及第二个季节性OMZ(Saanich Inlet),从而建立了比较基础,以描述上层硫化硫 - 硫代硫酸硫化硫磺及其在Omz Croniagnion and Impaction a emz Croniagnion Asignity As a ai Impaction Asiakins and Impaction a的生态分布。在多个学术层面上。将与佐治亚理工学院的K-12教育者和教师发展专家合作开发海洋科学(游泳)的夏季研讨会。为期5天的游泳计划,包括在Skidaway海洋学研究所举行的2天,将与研究生和佐治亚理工学院的研究人员培训当地教师,将海洋科学中的关键主题与中学地球科学教育中的新国家标准合并。此外,通过与莫尔豪斯(Morehouse)和斯佩尔曼(Spelman)学院的合作伙伴关系,该项目为暑期实习提供了资金,以增强少数族裔学生在本科生科学和生物信息学研究方面的代表。将在佐治亚理工学院建立一个PI-Gradure学生工作组,以制定和实施海洋基因组学跨学科领域培训的新准则。佐治亚理工学院将为科学家写作,以增强研究生的专业发展。它将使用结构化的,同行驱动的练习,使学生获得科学成功所必需的重要写作和口语技巧 - 佐治亚理工学院严重缺乏这样的课程。通过上面概述的活动,该职业项目不仅将建立一个研究小组,该研究小组致力于表征海洋微生物生态系统中具有生态意义但隐秘的组成部分,而且还将奠定强大的基础,PI可以通过该基础来利用他的工作结果来训练和激励子孙后代的海洋科学家。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Frank Stewart其他文献
As in Poland
就像在波兰一样
- DOI:
10.1353/man.2020.0063 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Yu Xiang;Ming Di;Frank Stewart - 通讯作者:
Frank Stewart
Living Spirit : Lierature and Resurgence in Oknawa
活生生的精神:奥克纳瓦的文学与复兴
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
Katsunori Yamazato;Frank Stewart - 通讯作者:
Frank Stewart
イギリス文化 55のキーワード
英国文化55个关键词
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
Katsunori Yamazato;Frank Stewart;野谷文昭;木下卓・窪田憲子・久守和子(共編著) - 通讯作者:
木下卓・窪田憲子・久守和子(共編著)
Une 《facon》 copiee-collee de l'Encyclopedie? : avatars de textes de l'HMARS a l'Enc.par l'intermediaire de E.Chambers
百科全书的《facon》副本:lHMARS 的文本头像和 E.Chambers 的中间人
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
Katsunori Yamazato;Frank Stewart;小磯花絵・伝康晴;寺田元一 - 通讯作者:
寺田元一
話者交替の精密なモデルに向けて:漸進的発話末予測モデルの提案
迈向说话者变化的精确模型:渐进式话语结束预测模型的提出
- DOI:
- 发表时间:
2010 - 期刊:
- 影响因子:0
- 作者:
Katsunori Yamazato;Frank Stewart;小磯花絵・伝康晴 - 通讯作者:
小磯花絵・伝康晴
Frank Stewart的其他文献
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{{ truncateString('Frank Stewart', 18)}}的其他基金
REU Site: Microbiology of Low Oxygen Ecosystems (MLOxE) at Montana State
REU 站点:蒙大拿州低氧生态系统 (MLOxE) 微生物学
- 批准号:
2349117 - 财政年份:2024
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
REU Site: Microbiology of Low Oxygen Ecosystems (MLOxE) at Montana State
REU 站点:蒙大拿州低氧生态系统 (MLOxE) 微生物学
- 批准号:
2051065 - 财政年份:2021
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
Collaborative Research: Nitrous oxide reduction in oxygen minimum zones: an understudied but critical loss term in ocean greenhouse gas cycling
合作研究:最低氧气区中的一氧化二氮还原:海洋温室气体循环中一个尚未充分研究但至关重要的损失项
- 批准号:
2022991 - 财政年份:2021
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss
合作研究:微多样性驱动生态系统功能:SAR11 细菌作为海洋氮流失的模型
- 批准号:
2130185 - 财政年份:2021
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
Microbial processes of pelagic anaerobic methane cycling in oxygen minimum zones
最低氧区中上层厌氧甲烷循环的微生物过程
- 批准号:
2054927 - 财政年份:2020
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
ABI Innovation: Collaborative Research: Computational framework for inference of metabolic pathway activity from RNA-seq data
ABI Innovation:协作研究:从 RNA-seq 数据推断代谢途径活性的计算框架
- 批准号:
1564559 - 财政年份:2016
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
Microbial processes of pelagic anaerobic methane cycling in oxygen minimum zones
最低氧区中上层厌氧甲烷循环的微生物过程
- 批准号:
1558916 - 财政年份:2016
- 资助金额:
$ 121.09万 - 项目类别:
Standard Grant
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