Plant Host Control and Feedback Responses to Phyllosphere Microbes: Change in Metagenomes and Plant Traits in Response to Environmental Stress

植物宿主对叶际微生物的控制和反馈反应：响应环境胁迫的宏基因组和植物性状的变化

• 批准号: 1126938
• 负责人: Ann Stapleton
• 金额: $ 60.15万
• 依托单位: University of North Carolina at Wilmington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1126938&HistoricalAwards=false
• 关键词: Plant; Host; Control; Feedback; Responses

PI: Ann E. Stapleton (University of North Carolina - Wilmington)CoPIs: Barbara Methé (J. Craig Venter Institute), Bradley W. Goodner (Hiram College), and Stuart G. Gordon (Presbyterian College)Plant growth and water relations can be affected by microbes on the leaf surface (the phyllosphere), either negatively through increased cuticle permeability or positively by increased water use efficiency. Plant leaf features thus interact with microbial characteristics and the abiotic environment to shape the adaptive landscape of the above-ground community. Limited water availability reduces maize (Zea mays L.) yield world-wide. Projections of crop effects under climate change indicate that water availability will decline, and water demand will increase. Maize leaf microbial community features are correlated with drought tolerance loci. The project will examine the association of phyllosphere microbes with maize drought responses using metagenomics to identify relevant functional genes. Better understanding of plant above-ground interactions with microbial inhabitants has the potential to fundamentally change breeding approaches for drought tolerance.The biology taught to high school students and undergraduates is increasingly distant from research biology. As a result, many of the best high-school students leave science behind as they choose college majors. Bridging these gaps is important to convince talented students that biology is relevant in their lives and that they can understand and be part of the scientific research enterprise. A decade ago genomics and bioinformatics started to provide integrated, "same-time" teaching/learning/research opportunities. Metagenomics is the next step up not just in terms of new research techniques and findings, but also in terms of getting students at multiple educational levels to connect genetics to ecology and physiology, and to better appreciate the roles of the vast diversity of microbes in natural and human altered habitats all over the planet. The project will build on past success in merging genomics research with education to include large metagenomics datasets. There is ample evidence from previous work that students can make significant research contributions. The project will develop the connections from the proposed experimental work to courses and develop cutting-edge functional tests compatible with undergraduate labs for biochemical activities emerging from the gene set found in the phyllosphere. All sequence data can be accessed at the NCBI Sequence Read Archive (SRA) and/or the European Bioinformatics Institute's European Nucleotide Archive (ENA). Metadata associated with the sequence reads and other types of data such as assemblies, annotation and metadata will also be deposited with the sequence reads as allowable through GenBank.

PI：Ann E. Stapleton（北卡罗来纳大学威尔明顿分校）CoPI：Barbara Methé（J. Craig Venter 研究所）、Bradley W. Goodner（海勒姆学院）和 Stuart G. Gordon（长老会学院）植物生长和水分关系可以受到叶子表面（叶际）微生物的影响，要么通过增加角质层渗透性产生负面影响，要么通过提高水分利用效率产生正面影响，从而与微生物特征和非生物相互作用。有限的可用水量会降低全球玉米（Zea mays L.）的产量。气候变化对作物影响的预测表明，可用水量将会下降，而玉米的需水量将会增加。叶子微生物群落特征与耐旱基因座相关，该项目将利用宏基因组学来研究叶际微生物与玉米干旱反应的关联，以识别相关的功能基因，从而有可能从根本上改变植物地上微生物的相互作用。向高中生和本科生教授的生物学与研究生物学越来越疏远，因此，许多最优秀的高中生在选择大学专业时放弃了科学，弥合这些差距非常重要。十年前，基因组学和生物信息学开始提供综合的、“同时”的教学/学习/研究机会。不仅仅是迈出一步不仅在新的研究技术和发现方面，而且还让不同教育水平的学生将遗传学与生态学和生理学联系起来，并更好地理解微生物在全球自然和人类栖息地中的巨大多样性的作用。该项目将建立在过去将基因组学研究与教育相结合的成功基础上，包括大型宏基因组学数据集。以前的工作有充分的证据表明，该项目将建立从拟议的实验工作到课程的联系，并开发前沿技术。边缘功能性与植物圈中发现的基因集的生化活性测试兼容的本科生实验室可以在 NCBI 序列读取档案 (SRA) 和/或欧洲生物信息学研究所的欧洲核苷酸元数据 (ENA) 中访问。序列读数和其他类型的数据（例如程序集、注释和元数据）也将在 GenBank 允许的情况下与序列读数一起存放。