Complete Reductive Dechlorination of Trichloroethylene (TCE) by non-Dehalococcoides Microorganisms

非脱卤球菌微生物对三氯乙烯 (TCE) 的完全还原脱氯

• 批准号: 1102889
• 负责人: Kevin Finneran
• 金额: $ 10.36万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1102889&HistoricalAwards=false
• 关键词: Complete; Reductive; Dechlorination; Trichloroethylene; TCE

ABSTRACTCBET-0853835Finneran, Kevin TUniversity of Illinois at Urbana-ChampaignComplete Reductive Dechlorination of Trichloroethylene (TCE) by non-Dehalococcoides MicroorganismsThe objectives of the proposed work are to: 1) demonstrate that organisms outside of the genus Dehalococcoides can completely reduce TCE to ethene, 2) use the enrichment cultures developed to characterize the metabolism, geochemistry, and chlorinated solvent biodegradation kinetics of non-Dehalococcoides microorganisms, 3) verify that the dominant non-Dehalococcoides microorganism(s) are most closely related to organisms within the genus Desulfosporosinus, 4) characterize the pathway for acetate oxidation and utilization during complete dechlorination to determine if it is directly oxidized as the sole electron donor (by Dehalococcoides or non-Dehalococcoides liquid enrichment cultures) or to define the mechanisms by which it may generate hydrogen under the same conditions, 5) demonstrate that electron donors (acetate or H2) added at stoichiometric concentrations relative to the electron acceptors present promotes faster TCE reduction to ethene when compared to excess electron donor addition, and 6) quantify the specific conditions in which Fe(III) reduction and complete dechlorination are promoted simultaneously.This is the first research to address the probability of non-Dehalococcoides complete dechlorination. This will change fundamental and applied science and engineering with respect to halorespiration as a metabolic process, and chlorinated solvent bioremediation as a practiced technology. In addition, the work will characterize several poorly understood environmental processes including the overlap of Fe(III) reduction and complete dechlorination, and acetate as a direct electron donor for halorespiration. The proposal has been constructed in a manner that all hypotheses are relevant and independent, and the data generated will benefit the broader scientific community studying basic halorespiration science or those investigating applied bioremediation of TCE.The proposed outreach plan incorporates both students who are members of underrepresented groups in environmental engineering, and practitioners who will ultimately benefit from the data. The students will be both graduate level and undergraduate level, and will be recruited specially from programs for minority and female students. The practitioner aspect will culminate in direct contact with one group who has already expressed interest in the work and with others via conferences and individual presentations to small groups at their regional offices. Entire research staff including the proposed postdoctoral scholar will interact with all practitioners such that they become accustom to the lab to field transition for research, and how the pragmatic aspects of environmental engineering practice can influence fundamental research science.

AbstractCBET-0853835FINNERAN，伊利诺伊州的Kevin Tumiversity在urbana-champaignComplete上还原二氯乙烯（TCE）的脱氯苯基（TCE），由非脱霍洛甘烯二氯乙烯微生物造成的微生物ssssssssss s的目标是：1）完全降低了2）的用途：1）完全降低了Dehalocco的用途。开发出旨在表征代谢，地球化学和氯化溶剂生物降解动力学动力学的富集培养物，3）验证，验证主要的非脱核苷的微生物微生物微生物（S）与均具有氧化型和氧化型的有机化相关性，供体现型和蛋白酶孔素化。 during complete dechlorination to determine if it is directly oxidized as the sole electron donor (by Dehalococcoides or non-Dehalococcoides liquid enrichment cultures) or to define the mechanisms by which it may generate hydrogen under the same conditions, 5) demonstrate that electron donors (acetate or H2) added at stoichiometric concentrations relative to the electron acceptors present promotes faster TCE reduction to ethene when compared对于过量的电子供体添加和6）量化了同时促进Fe（III）降低和完全脱氯的特定条件。这是解决非脱甲基胶菌完全脱氯的可能性的首次研究。这将改变基本和应用的科学和工程，以卤索作为代谢过程，将氯化溶剂生物修复作为实践技术。此外，这项工作将表征几个鲜为人知的环境过程，包括Fe（III）降低和完全脱氯的重叠，以及乙酸盐作为Halorespiration的直接电子供体。该提案的构建方式是所有假设都是相关且独立的，并且产生的数据将使研究基本呼吸科学的更广泛的科学社区或研究对TCE的应用生物修复的人。拟议的外展计划融合了两个学生都是在环境工程中占人不足的群体成员的成员，这些学生最终将受益于数据。学生将既是研究生级别又是本科生，并将特别是从少数民族和女学生的计划中招募的。从业人员方面将直接与一个已经通过会议和个人演讲向其区域办事处的小组进行了对工作的兴趣和其他人的兴趣直接接触。包括拟议的博士后学者在内的整个研究人员将与所有从业人员进行互动，以使他们习惯于实验室进行研究，以进行研究，以及环境工程实践的实用方面如何影响基本的研究科学。