RUI: Mechanism of Antibiotic Resistance Associated with the Arabidopsis ABC Protein, Atwbc19

RUI：与拟南芥 ABC 蛋白 Atwbc19 相关的抗生素耐药性机制

• 批准号: 1052172
• 负责人: Mentewab Ayalew
• 金额: $ 37.07万
• 依托单位: Spelman College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1052172&HistoricalAwards=false
• 关键词: RUI; Mechanism; Antibiotic; Resistance; Associated

INTELLECTUAL MERIT ABC (ATP binding cassette ) proteins represent a gene family that has greatly expanded in plants, with twice as many members in Arabidopsis and rice (over 120) as any other sequenced genome of a multicellular organism (eg. human, worm, fly). The reason behind the expansion of this family in plants is unknown but hints to kingdom specific evolutionary constraints. This project focuses on the Arabidopsis ABC protein, Atwbc19 (ABCG19), a member of a plant-specific clade. Atwbc19 confers antibiotic resistance but the underlying mechanism is not clear. Preliminary results suggest that Atwbc19 does not function as a transporter but as a translational regulator. Thus the goal of this project is to demonstrate cleavage of Atwbc19 resulting in a soluble ABC protein, confirm its association with the translation apparatus and identify regulated transcripts. Ultimately, a new paradigm for antibiotic resistance in plants may be established.BROADER IMPACT The project has three educational objectives. First, long term research opportunities will be provided to undergraduates. Second, a research based course will be developed. And finally, research experiences will be provided in the traditional classroom through an upper level Plant Biology course. Overall, the project will contribute to the strategic goal of the College to increase student participation in research or internships from 30% to 100% by 2015. At the departmental level, the project will contribute to a curriculum that is being significantly revised towards a stronger emphasis on linking research to teaching. Ultimately, one goal is to inspire African American female students to engage in intellectual and personal development that leads them to Graduate School.

智力价值 ABC（ATP 结合盒）蛋白代表了一个在植物中极大扩展的基因家族，其在拟南芥和水稻中的成员数量（超过 120 个）是多细胞生物（例如人类、蠕虫、苍蝇）的任何其他已测序基因组的两倍）。该家族在植物中扩张的原因尚不清楚，但暗示了王国特定的进化限制。 该项目重点关注拟南芥 ABC 蛋白 Atwbc19 (ABCG19)，它是植物特异性进化枝的成员。 Atwbc19 赋予抗生素耐药性，但其潜在机制尚不清楚。初步结果表明，Atwbc19 的功能不是转运蛋白，而是翻译调节因子。因此，该项目的目标是证明 Atwbc19 的裂解产生可溶性 ABC 蛋白，确认其与翻译装置的关联并鉴定受调控的转录本。最终，可能会建立植物抗生素抗性的新范例。 更广泛的影响 该项目有三个教育目标。首先，将为本科生提供长期的研究机会。 其次，将开发基于研究的课程。最后，将通过高级植物生物学课程在传统课堂上提供研究经验。总体而言，该项目将为学院的战略目标做出贡献，即到 2015 年将学生参与研究或实习的比例从 30% 提高到 100%。在院系层面，该项目将有助于对课程进行重大修订，以实现更强大的目标。强调研究与教学相结合。最终，目标之一是激励非裔美国女学生进行智力和个人发展，引导她们进入研究生院。