RUI: Mechanism of Antibiotic Resistance Associated with the Arabidopsis ABC Protein, Atwbc19

RUI：与拟南芥 ABC 蛋白 Atwbc19 相关的抗生素耐药性机制

• 批准号: 1052172
• 负责人: Mentewab Ayalew
• 金额: $ 37.07万
• 依托单位: Spelman College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1052172&HistoricalAwards=false
• 关键词: RUI; Mechanism; Antibiotic; Resistance; Associated

INTELLECTUAL MERIT ABC (ATP binding cassette ) proteins represent a gene family that has greatly expanded in plants, with twice as many members in Arabidopsis and rice (over 120) as any other sequenced genome of a multicellular organism (eg. human, worm, fly). The reason behind the expansion of this family in plants is unknown but hints to kingdom specific evolutionary constraints. This project focuses on the Arabidopsis ABC protein, Atwbc19 (ABCG19), a member of a plant-specific clade. Atwbc19 confers antibiotic resistance but the underlying mechanism is not clear. Preliminary results suggest that Atwbc19 does not function as a transporter but as a translational regulator. Thus the goal of this project is to demonstrate cleavage of Atwbc19 resulting in a soluble ABC protein, confirm its association with the translation apparatus and identify regulated transcripts. Ultimately, a new paradigm for antibiotic resistance in plants may be established.BROADER IMPACT The project has three educational objectives. First, long term research opportunities will be provided to undergraduates. Second, a research based course will be developed. And finally, research experiences will be provided in the traditional classroom through an upper level Plant Biology course. Overall, the project will contribute to the strategic goal of the College to increase student participation in research or internships from 30% to 100% by 2015. At the departmental level, the project will contribute to a curriculum that is being significantly revised towards a stronger emphasis on linking research to teaching. Ultimately, one goal is to inspire African American female students to engage in intellectual and personal development that leads them to Graduate School.

智力功绩ABC（ATP结合盒）蛋白代表了一种基因家族，在植物中大大扩展，拟南芥和大米的成员（超过120）是多细胞生物的任何其他测序基因组的两倍（例如，人类，蠕虫，飞蝇）。该家族在植物中扩张的原因尚不清楚，但暗示了王国特定的进化约束。 该项目着重于拟南芥ABC蛋白ATWBC19（ABCG19），这是植物特异性进枝的成员。 ATWBC19赋予抗生素耐药性，但基本机制尚不清楚。初步结果表明，ATWBC19不充当转运蛋白，而是转化调节剂。因此，该项目的目的是证明ATWBC19的裂解，从而产生可溶性ABC蛋白，确认其与翻译设备的关联并识别受调节的转录本。最终，可以建立一种新的抗生素耐药性范式。该项目具有三个教育目标。首先，将向大学生提供长期研究机会。 其次，将开发基于研究的课程。最后，将通过上层植物生物学课程在传统教室中提供研究经验。总体而言，该项目将有助于大学的战略目标，以将学生参与研究或实习从2015年提高30％。最终，一个目标是激发非洲裔美国女学生从事智力和个人发展，使他们进入研究生院。