Quantification of the Biotransport Phenomena in Low-Cryoprotectant (CPA) Vitrification of Living Cells

活细胞低冷冻保护剂 (CPA) 玻璃化冷冻中生物转运现象的定量

• 批准号: 1033426
• 负责人: Xiaoming He
• 金额: $ 30万
• 依托单位: University South Carolina Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1033426&HistoricalAwards=false
• 关键词: Quantification; Biotransport; Phenomena; Low; Cryoprotectant

1033426HeContemporary methods for cell cryopreservation often produce a low yield of viable cells due to the harmful effects of ice formation or use of a high concentration of cryoprotectant (CPA). This research seeks to achieve ice-free cryopreservation (vitrification) of cells using a low, nontoxic concentration of CPA (low-CPA). A quantitative understanding of the thermally driven, non-equilibrium phase transition of water in living cells into either a crystalline or a glassy solid, and its correlation with cell survival during low-CPA vitrification has not been achieved to date. Therefore, the objective of this project is to quantify the relevant fundamental phenomena by model development coupled with experimental verification.Intellectual Merit: A coupled thermal and phase transition model will be developed which will facilitate quantitative understanding of the pertinent biotransport processes during low-CPA vitrification. Optimal low-CPA vitrification protocols for cryopreserving various living cells will be determined. Broader Impacts: The proposed research will assist the advancement of emerging cell-based medicine of which the eventual success relies upon the capability to preserve living cells for distribution to end users. Moreover, the proposed research will be integrated into education through (a) curriculum development, (b) training of graduate, undergraduate, and high school students, and (c) dissemination of the research findings including by a specialized web page.

细胞冷冻保存的1033426经久方法通常由于冰的有害作用或使用高浓度的冷冻保护剂（CPA）而产生的活细胞产量低。这项研究旨在使用低毒性的CPA（低CPA）实现细胞无冰的冷冻保存（玻璃化）。对活细胞中水的热驱动的非平衡相过渡到晶体或玻璃固体的定量理解，并且迄今尚未实现其与细胞存活期间的相关性。因此，该项目的目的是通过模型开发以及实验验证来量化相关的基本现象。IntlectualFure：将开发一个耦合的热和相变模型，这将促进对低CPA玻璃体期间对相关生物转移过程的定量理解。将确定用于冷冻的各种活细胞的最佳低CPA玻璃化方案。更广泛的影响：拟议的研究将有助于发展新兴细胞的药物，最终成功依赖于将活细胞保存到最终用户的能力。此外，拟议的研究将通过（a）课程开发，（b）培训研究生，本科和高中生，以及（c）传播研究结果，包括专业网页，包括专业网页。