Applying isotachophoresis for transporting, contacting, and separating proteins, DNA, and particles

应用等速电泳来运输、接触和分离蛋白质、DNA 和颗粒

• 批准号: 198842835
• 负责人: Professor Dr. Steffen Hardt
• 金额: --
• 依托单位: Fachgebiet Nano- und Mikrofluidik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/198842835?language=en
• 关键词: Applying; isotachophoresis; transporting; contacting; separating

The overarching goal of the proposal is to explore novel application areas for microchannel isotachophoresis (ITP). In that context, two different approaches will be followed. On the one hand it should be studied how far steep gradients of ionic surfactants that are created with the help of ITP may be exploited to separate small particles, especially nanoparticles. Particles at an ITP interface should experience surface forces in such concentration gradients. In addition to that, the concentration of an ionic surfactant should also influence the electrophoretic mobility of a particle, yielding different values in front of and behind the ITP interface. The interaction of small particles with ITP interfaces exhibiting a steep concentration gradient of an ionic surfactant will be studied based on fluorescence microscopy. On the other hand, it is planned to study enzymatic reactions with proteins concentrated in ITP zones. Since that way the protein concentration can be increased by many orders of magnitude compared to standard protocols, it can be speculated that enzymatic reactions can be speeded up significantly. In addition to that, since the required amounts of enzyme and substrate are very small, corresponding protocols should be ideally suited for applications in the field of high-throughput screening.

该提案的总体目标是探索微通道同疗法的新型应用领域（ITP）。在这种情况下，将遵循两种不同的方法。一方面，应该研究在ITP帮助下产生的离子表面活性剂的陡峭梯度，可能会被利用为分离的小颗粒，尤其是纳米颗粒。 ITP界面处的颗粒应在这种浓度梯度中经历表面力。除此之外，离子表面活性剂的浓度还应影响粒子的电泳迁移率，在ITP界面前后产生不同的值。基于荧光显微镜，将研究小颗粒与ITP界面表现出陡峭浓度梯度的相互作用。另一方面，计划研究浓缩ITP区域的蛋白质的酶促反应。由于与标准方案相比，由于这种方式可以通过许多数量级增加蛋白质浓度，因此可以推测可以显着加快酶促反应。除此之外，由于所需量的酶和底物非常小，因此相应的协议应适合于高通量筛选领域的应用。

项目成果

Increasing the sensitivity of microfluidics based immunoassays using isotachophoresis.

• DOI: 10.1039/c4an00545g
• 发表时间: 2014-08
• 期刊: The Analyst
• 作者: R. Khnouf;Gabriele Goet;T. Baier;S. Hardt