Collaborative Research: The Contribution of Polyamines to N and C cycling in Marine Systems

合作研究：多胺对海洋系统中 N 和 C 循环的贡献

基本信息

批准号：
1029742
负责人：
James Hollibaugh
金额：
$ 18.99万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2010
资助国家：
美国
起止时间：
2010-10-01 至 2013-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1029742&HistoricalAwards=false
关键词：
Collaborative Research Contribution Polyamines cycling

项目摘要

While dissolved organic nitrogen (DON) plays a central role in global ocean N cycling, little is known about specific DON components. To address one aspect of this, investigators from Kent State University and the University of Georgia will examine polyamines, an under-studied microbially-produced DON component. They hypothehesize that, despite the low concentrations of these N-rich compounds, free polyamines have an important role in microbial ecology. To test this hypothesis, the investigators will examine the concentration, flux and fate of dissolved polyamines and dissolved amino acids at nearshore and open ocean sites using various chemical analyses. The composition of bacterial assemblages and of genes involved in polyamine utilization will also be determined using state-of-the-art molecular techniques. By integrating biogeochemical and molecular approaches, and linking functional genomicsof plankton assemblages to material fluxes, the work will improve the understanding of the fate and dynamics of DON in marine environments, and the importance of polyamines as sources of C and N. This collaborative project willenhance inter-institution partnerships, and train graduate students while alsoreaching out to under-represented and promising first- andsecond-year undergraduate and high school students. The students will be involved with research opportunities, summer internships, and other activities.

尽管溶解的有机氮（DON）在全球海洋N循环中起着核心作用，但对特定的DON组件知之甚少。为了解决此方面的一个方面，肯特州立大学和佐治亚大学的研究人员将检查多胺（一种生产不足的微生物生产的DON组件）。他们假设，尽管这些富含N的化合物浓度低，但游离多胺在微生物生态学中具有重要作用。为了检验这一假设，研究人员将使用各种化学分析检查溶解多胺和溶解的氨基酸的浓度，通量和命运。还将使用最先进的分子技术确定细菌组合物和参与多胺利用的基因的组成。通过整合生物地球化学和分子方法，并将浮游生物组合的功能性基因组与物质通量联系起来，这项工作将改善对海洋环境中DON的命运和动态的理解，以及多胺作为C和N的重要性。以及二年级的本科生和高中生。学生将参与研究机会，暑期实习和其他活动。