EAGER: Isolation of ionizing radiation resisting microorganisms from the Gamma Forest in Long Island, NY

EAGER：从纽约长岛伽马森林中分离出抗电离辐射微生物

• 批准号: 1028438
• 负责人: Vishal Shah
• 金额: --
• 依托单位: Dowling College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1028438&HistoricalAwards=false
• 关键词: EAGER; Isolation; ionizing; radiation; resisting

Principal Investigator: Vishal ShahProposal No: CBET- 1028438 Over the period of the last few years, it has been realized that a potential exists to enrich lighter uranium and plutonium from the high-level radioactive waste. The PI?s long term goal is to develop a microorganism based technology that could carry out such enrichment. In the current proposal he aims to isolate microorganisms having resistance to high intensity ionizing radiation. The soil of Gamma Forest, located at the Brookhaven National Laboratory, will be used to screen the organisms having desired properties. Gamma Forest was a radiation facility established in 1961 to provide opportunity for systematic study of the effects of ionizing radiation on a terrestrial ecosystem and its components. The source of radiation used during the experiment was cesium-137 (9500 curies), a gamma emitter. In the study, the radiation source was placed on a tower located in the center of the field and the field was exposed 20 hours/day daily till 1979. Rates of exposure around the source varied from several thousand roentgens per day within a few meters of the source to about 2 roentgens per day at 130 meters. The working hypothesis for the proposed research is that, based on the intensity of radiation that the soil was exposed at various distances from Cs137, different types of ionizing radiation resisting microorganisms would have survived. The experimental approach involves exposing the soil obtained from various locations of gamma forest to gamma radiation. The microorganisms from soil would then be isolated using serial dilution and plating approaches. The resistance to ionizing radiation will be confirmed by again exposing the cultures to gamma radiation. All the isolated cultures having resistance to radiation will be identified using molecular methods.The proposed research will impact the disciplines of Environmental Sciences, Microbiology and Biotechnology: 1. New extremophilic microorganisms that are acidophilic/acid tolerant and radiation tolerant could be expected to be isolated in the proposed research. These cultures could have multiple applications in the field of biotechnology and environmental sciences. 2. The research would expand the library of gram negative, gram positive, yeast and fungi cultures in Biolog and 16S rDNA database. This would help scientists working in similar areas to identify the cultures which would be added in the database through this project with ease.The proposed project will impact science in a broader sense by: 1. The student who will be trained through this project will have an in depth research experience in classical microbiology and be better positioned to contribute to novel concepts in modern microbial techniques. 2. The project reinforces the educational experience for a socially diverse and economically disadvantaged student body, including those from underrepresented groups in the New York City area. At Dowling College, underrepresented minorities are close to 50% of the student population, and there are 70% females in the Department of Biology at Dowling College. 3. Also, instruments obtained through the funding of this project will be used in conducting new laboratory experiments in Introduction to Biology, Microbiology and Biochemistry courses. This will aid in developing more scientifically trained youth for our society.

首席研究人员：Vishal Shahproposso No：CBET-1028438在过去几年中，人们已经意识到存在潜在的潜在，可以从高级放射性废物中富集更轻的铀和plut。 PI的长期目标是开发基于微生物的技术，该技术可以进行这种富集。 在当前的建议中，他旨在分离具有对高强度电离辐射的抗性的微生物。 位于布鲁克黑文国家实验室的伽马森林的土壤将用于筛选具有所需特性的生物。 伽马森林是一种辐射设施，成立于1961年，旨在系统地研究电离辐射对陆地生态系统及其组件的影响。 实验过程中使用的辐射来源是颅137（9500鼠），一个伽马发射器。 在研究中，将辐射源放在位于田野中心的塔上，每天每天20小时暴露于1979年。源周围的暴露率从源头几米的每天几千五千五千米到每天约2个Roentgens到130米的每天约2个Roentgens。 拟议研究的工作假设是，基于辐射的强度，土壤在与CS137的不同距离处暴露的强度，不同类型的电离辐射抗微生物将存活。 实验方法涉及暴露从伽马森林各个位置获得的土壤到伽马辐射。 然后，将使用连续稀释和镀层方法分离来自土壤的微生物。 通过再次将培养物暴露于伽马辐射，将确认对电离辐射的抗性。 所有具有抗辐射能力的孤立培养物将使用分子方法鉴定。拟议的研究将影响环境科学，微生物学和生物技术的学科：1。在拟议的研究中预计可以隔离于嗜酸性/酸性耐酸/酸耐酸和辐射耐受性。这些文化可能在生物技术和环境科学领域具有多种应用。 2。该研究将扩大生物学和16S rDNA数据库中革兰氏阴性，革兰氏阴性，酵母和真菌培养物的库。 这将有助于在类似领域工作的科学家通过该项目轻松识别数据库中添加的文化。拟议的项目将在更广泛的意义上影响科学：1。将通过该项目进行培训的学生在经典微生物学方面具有深入的研究经验，并在现代微生物技术中为新颖的概念做出了更好的作用。 2。该项目增强了社会多元化和经济不利的学生的教育经验，包括来自纽约市地区代表性不足的群体的教育经验。 在道林学院，代表性不足的少数民族接近学生人数的50％，而道林学院的生物学系有70％的女性。 3。此外，通过该项目的资助获得的工具将用于在生物学，微生物学和生物化学课程中进行新的实验室实验。 这将有助于为我们的社会发展更科学培训的青年。