Isotope studies of microbial sulfate reduction

微生物硫酸盐还原的同位素研究

• 批准号: 1021762
• 负责人: James Farquhar
• 金额: $ 3万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1021762&HistoricalAwards=false
• 关键词: Isotope; studies; microbial; sulfate; reduction

This proposal requests funds to support an experimental study of S isotope fractionation by sulfate reducing organisms, coupling well-established methods for measuring the fractionation of four stable isotopes of sulfur with experiments involving 17O isotope-labeling techniques. The project aims to explore the response of these organisms to temperature and electron donors. The work will involve sulfur isotope analysis of samples from culture experiments undertaken prior to submission of the proposal as part of the first part of a significantly larger project to study the sulfate reduction metabolism. It is anticipated that the support will lead to the publication of a paper on the metabolic response of Desulfobacterium autotrophicum to changes in the electron donor at two temperature conditions.Understanding the sedimentary sulfur cyle and the catalyses by mircobes is fundamental for understanding the global carbon cycle. The proposal will provide partial support for this work in the form of several months of support for a graduate student and partial support for analytical costs.

该提案请求资金支持硫酸盐还原生物对硫同位素分馏的实验研究，将测量四种稳定硫同位素分馏的成熟方法与涉及 17O 同位素标记技术的实验相结合。该项目旨在探索这些生物体对温度和电子供体的反应。这项工作将涉及对提交提案之前进行的培养实验样品进行硫同位素分析，作为研究硫酸盐还原代谢的更大项目的第一部分的一部分。预计该支持将导致发表一篇关于自养脱硫杆菌在两种温度条件下对电子供体变化的代谢反应的论文。了解沉积硫循环和微生物的催化是了解全球碳循环的基础。该提案将以对研究生几个月的支持和对分析费用的部分支持的形式为这项工作提供部分支持。