Collaborative Research: Arabidopsis 2010: Dissecting Cortical Actin Function during Arabidopsis-Pseudomonas Interactions

合作研究：拟南芥 2010：剖析拟南芥-假单胞菌相互作用期间的皮质肌动蛋白功能

• 批准号: 1021463
• 负责人: Jeffrey Chang
• 金额: $ 30.04万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1021463&HistoricalAwards=false
• 关键词: Collaborative; Research; Arabidopsis; 2010; Dissecting

An exquisite and intricate crosstalk exists between bacterial phytopathogens and the host cells and tissues they invade. New information suggests that a dynamic network of filaments, the cytoskeleton, is essential for both resistance and susceptibility to plant pathogens. However, exactly which molecules from the bacterium stimulate cytoskeletal responses, and which plant proteins are necessary for these responses remains poorly understood. In this project, the investigators will dissect the genetic basis for crosstalk between bacterial effector proteins and the plant host cytoskeleton. Using both molecular genetics tools and advanced imaging technologies, the investigators will test the hypothesis that specific pathogen effector proteins perturb plant actin-binding proteins (ABPs), which in turn leads to alterations in the plant cytoskeleton during defense signaling. A toolbox of actin and actin-binding protein mutants will be developed, and a delivery system for introducing bacterial effectors into host cells will be exploited. Through this work, the PIs aim to identify the function of bacterial effector proteins and 39 plant genes/proteins in order to uncover the signaling network. Lead PI Staiger will coordinate activities between the groups and exploit state-of-the-art imaging modalities to evaluate the defects in actin dynamics in the ABP collection and in response to effector proteins. The Day and Chang laboratories will use genetic approaches to dissect whether crosstalk involves pathogen-associated molecular pattern recognition responses (PTI) or effector-triggered immunity (ETI), or both. These two labs will also use a novel bacterial delivery system to associate changes in actin dynamics to a corresponding bacterial effector protein. The two labs will also prepare constructs for protein-protein interaction assays and subcellular localization. All project data and resources created in this work will be disseminated in a timely fashion at a joint website: http://actinpathogen-network.msu.edu. The broader impact of this work will contribute to a deep understanding of host?pathogen signaling and will enable crop modifications to protect against diseases that devastate agricultural productivity. As a unique outreach component, we will establish a summer workshop to train all project scientists and other interested parties, including local high school students, in advanced methods for imaging cellular dynamics.

细菌植物病原体与它们入侵的宿主细胞和组织之间存在精致而复杂的串扰。新信息表明，细丝的动态网络是细胞骨架，对于抗植物病原体的耐药性和易感性都是必不可少的。但是，从细菌中的哪些分子确切地刺激细胞骨架反应，以及这些植物蛋白对于这些反应所必需的植物蛋白仍然知之甚少。在该项目中，研究人员将在细菌效应蛋白和植物宿主细胞骨架之间剖析遗传基础。使用分子遗传学工具和高级成像技术，研究人员将检验以下假设：特定病原体效应蛋白会扰动植物肌动蛋白结合蛋白（ABP），这又导致防御信号期间植物细胞骨架的改变。将开发肌动蛋白和肌动蛋白结合蛋白突变体的工具箱，并将利用用于将细菌效应子引入宿主细胞的输送系统。通过这项工作，PIS旨在确定细菌效应蛋白和39个植物基因/蛋白质的功能，以发现信号网络。铅Pi Staiger将协调两组之间的活动，并利用最先进的成像方式，以评估ABP收集中肌动蛋白动力学中的缺陷并响应效应蛋白。日子和跨性实验室将使用遗传方法来剖析串扰是涉及病原体相关的分子模式识别反应（PTI）还是效应子触发的免疫（ETI），还是两者兼有。这两个实验室还将使用新型的细菌递送系统将肌动蛋白动力学的变化与相应的细菌效应蛋白相关联。这两个实验室还将为蛋白质蛋白质相互作用测定和亚细胞定位准备构造。在这项工作中创建的所有项目数据和资源都将在联合网站上及时传播：http：//actinpathogen-network.msu.edu。这项工作的更广泛的影响将有助于对宿主的深刻理解？病原体信号传导，并能够改变作物以防止破坏农业生产力的疾病。作为独特的外展部分，我们将建立一个夏季研讨会，以培训所有项目科学家和其他有关方面，包括当地高中生，以成像蜂窝动力学的高级方法。