Genomic dissection of floral transition in Brassica napus towards crop improvement by life cycle adaptation and hybrid yield increase

通过生命周期适应和提高杂交产量，对甘蓝型油菜花向作物改良的转变进行基因组解剖

• 批准号: 197249652
• 负责人: Professor Dr. Christian Jung
• 金额: --
• 依托单位: Lehrstuhl Pflanzenzüchtung
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/197249652?language=en
• 关键词: Genomic; dissection; floral; transition; Brassica

Rapeseed (Brassica napus L.) suffers from low genetic variation due to the short history of this species. Breeders try to broaden the genetic basis by gene introgression from non-adapted material from other geographic regions of the world. However, use of these materials is hampered, among others, by non-adapted flowering time (FTi). Here an integrated project is proposed to get a deeper understanding of FTi by global expression analysis and cloning of major FTi regulators. Candidate genes will be mapped by recombination mapping and, in collaboration with other groups, by association mapping. As a proof of concept study, relevant sequences will be mapped to recombinant lines carrying exotic rapeseed introgressions. The 2nd part of the project will study the relevance of 4 FTi genes for heterosis. Assuming that sequence variation within these genes will have an impact on seed yield and biomass heterosis, mutants will be identified by TILLING. The mutants will be analyzed and crosses will be made to determine heterosis of F1 hybrids in the 2nd funding period.

油菜籽 (Brassica napus L.) 由于该物种历史较短，因此遗传变异较低。育种者试图通过来自世界其他地理区域的未适应材料的基因渗入来扩大遗传基础。然而，这些材料的使用受到不适应的开花时间（FTi）等因素的阻碍。这里提出了一个综合项目，通过主要 FTi 调节因子的全局表达分析和克隆来更深入地了解 FTi。候选基因将通过重组作图以及与其他小组合作通过关联作图来进行图谱绘制。作为概念研究的证明，相关序列将被映射到携带外来油菜籽基因渗入的重组品系。该项目的第二部分将研究 4 个 FTi 基因与杂种优势的相关性。假设这些基因内的序列变异将对种子产量和生物量杂种优势产生影响，则将通过 TILLING 来鉴定突变体。在第二个资助期内，将分析突变体并进行杂交以确定 F1 杂种的杂种优势。

项目成果

Mutations in single FT- and TFL1-paralogs of rapeseed (Brassica napus L.) and their impact on flowering time and yield components

油菜籽 (Brassica napus L.) 单一 FT- 和 TFL1-旁系同源物的突变及其对开花时间和产量构成的影响

• DOI: 10.3389/fpls.2014.00282
• 发表时间: 2014
• 期刊: Frontiers in Plant Science
• 影响因子: 5.6
• 作者: Guo Y;Hans H;Christian J;Molina C
• 通讯作者: Molina C