MRI-R2: Development of High-Resolution Single Fluorescent Particle Tracker and Nanomanipulator

MRI-R2：高分辨率单荧光粒子追踪器和纳米操纵器的开发

• 批准号: 0959823
• 负责人: Nils Walter
• 金额: $ 116.3万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-15 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959823&HistoricalAwards=false
• 关键词: MRI; R2; Development; Resolution; Single

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of two versatile, modular fluorescence microscopy platforms for tracking and mechanically probing single molecules/particles at the University of Michigan (UM), and the installation of a third, user-friendly microscope system for user training and project development. The development and application of two open-access single molecule tracking and probing microscopes transforms the limited capability for high-content screening of biological samples. Benchmarks are set that make both instruments operational within two years and lay out plans for testing, further optimization and expansion over an additional two years, after which recharges will maintain them. The instruments are being utilized for research and education by the University of Michigan community and beyond, engaging physical and biological scientists as well as an engineer. This development activity helps establish a unique, nation-wide, educational Center of Single Molecule Analysis (CoSiMA) in a highly cross-disciplinary, emerging field of transformative power in the biosciences. CoSiMA is engaging in vigorous outreach efforts to experimentalists, theorists, undergraduates, and high school students with large, local, underrepresented groups, as well as the public at large, in close collaboration with UM's existing IDEA Institute. These efforts are facilitated by the creation of a central laboratory facility with access to multiple single molecule microscopes under guidance of a Steering Committee and a senior staff scientist, as well as by the broad inspirational appeal of the emerging single molecule biosciences. Results from the studies enabled by the new equipment are disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.

该奖项根据 2009 年美国复苏和再投资法案（公法 111-5）提供资金。 这项重大研究仪器回收和再投资 (MRI-R2) 奖项资助密歇根大学 (UM) 开发两个多功能模块化荧光显微镜平台，用于跟踪和机械探测单分子/颗粒，并安装第三个平台，用户友好的显微镜系统，用于用户培训和项目开发。两种开放式单分子跟踪和探测显微镜的开发和应用改变了生物样品高内涵筛选的有限能力。设定的基准使这两种仪器在两年内投入运行，并制定了在另外两年内进行测试、进一步优化和扩展的计划，之后充电将维持它们。这些仪器被密歇根大学社区及其他机构用于研究和教育，吸引了物理和生物科学家以及工程师。 这项开发活动有助于在生物科学的高度跨学科、新兴的变革力量领域建立一个独特的全国性单分子分析教育中心 (CoSiMA)。 CoSiMA 正在与 UM 现有的 IDEA 研究所密切合作，积极向实验家、理论家、本科生和高中生以及大型、当地、代表性不足的群体以及广大公众进行推广。在指导委员会和一名高级科学家的指导下，建立了一个可以使用多个单分子显微镜的中央实验室设施，以及新兴的单分子生物科学的广泛鼓舞人心的吸引力，促进了这些努力。 新设备的研究结果通过学生和教师在地区和国家会议上的演讲以及在同行评审期刊上发表来传播。