Collaborative Research: Plant heat tolerance through cross-kingdom interactions

合作研究：通过跨界相互作用实现植物耐热性

• 批准号: 0950447
• 负责人: Russell Rodriguez
• 金额: $ 52万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2014-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0950447&HistoricalAwards=false
• 关键词: Collaborative; Research; Plant; heat; tolerance

Most plants cannot grow when soil temperatures reach levels that are above 100 °F. However, in Yellowstone National Park soil temperatures can be much higher in the geothermal areas, and occasionally plants can be found growing in soils in temperatures up to 125 °F. These plants have a fungus, called Curvularia protuberata that grows in them, and they cannot grow in these soil temperatures without the fungus. The fungus itself is infected with a virus, and the virus also is required for the whole thermal tolerant system of plant, fungus and virus (called a symbiosis) to survive. However, the interactions between the virus and fungus in this system and other microbe-interacting systems in the environment are very poorly understood. A crucial key to the understanding of adaptive changes in response to environmental stress is missing. In addition, very few beneficial viruses have been described or studied. The long-term goal of the research is to understand how plants and mutualistic microorganisms interact with one another and their surrounding environments to acquire stress tolerance. This project will study the physiology and genetics that define the mechanism of this interaction. Its goals are to: 1) Identify and characterize additional plant-fungus-virus symbionts that are thermotolerant; 2) Identify the genetic expression and metabolite changes of plants and fungi involved in the three way symbiosis during heat stress; 3) Study how the proteins from each organism interact; and 4) Test the genes discovered for their effects on tolerance to heat. With changes in the Earth's climate and the need to produce more and more food for the increasing human population, learning how plants adapt naturally to environmental changes such as are seen in Yellowstone National Park will provide tools will potential application to agriculture.

当土壤温度达到高于100°F的水平时，大多数植物无法生长。但是，在黄石国家公园中，地热区的土壤温度可能更高，偶尔会在最高125°F的温度下发现植物在土壤中生长。这些植物的真菌被称为曲线蛋糕，它们在其中生长，并且在没有真菌的情况下，它们在这些土壤温度中无法生长。真菌本身被病毒感染，并且整个植物，真菌和病毒（称为象征意义）的整个热耐受性系统也需要该病毒才能生存。但是，该系统中病毒与真菌之间的相互作用与环境中的其他微生物相互作用系统的相互作用非常差。缺少对环境压力的自适应变化理解的关键关键。此外，很少描述或研究了很少的有益病毒。这项研究的长期目标是了解植物和相互主义的微生物如何相互相互作用及其周围环境以获得胁迫耐受性。该项目将研究定义这种相互作用机制的生理和遗传学。它的目标是：1）识别和表征耐热剂的其他植物 - 脉冲病毒象征； 2）确定热应激过程中植物和真菌涉及的植物和真菌的遗传表达和代谢物变化； 3）研究每个生物体的蛋白质如何相互作用； 4）测试发现其对热耐受的影响的基因。随着地球气候的变化以及为增加人口生产越来越多的食物的需求，学习植物如何自然地适应了黄石国家公园中所见的环境变化，这将为农业提供潜在的应用。