MRI-RUI: Acquisition of a Spinning confocal Microscope to Advance Interdepartmental Research and Training at Missouri State University-Springfield

MRI-RUI：购买旋转共焦显微镜以推进密苏里州立大学斯普林菲尔德分校的跨部门研究和培训

• 批准号: 0923024
• 负责人: Kyoungtae Kim
• 金额: $ 24.84万
• 依托单位: Missouri State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923024&HistoricalAwards=false
• 关键词: MRI; RUI; Acquisition; Spinning; confocal

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).A grant has been awarded to Missouri State University (MSU) to acquire a laser spinning confocal fluorescence microscope equipped with a Yokogawa spinning confocal scanner and four laser lines for research and teaching at the university and in the region. This instrument will not only allow researchers to achieve significantly improved quality in microscopic imaging but will also afford them the opportunity to perform advanced techniques such as fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), optical sectioning of specimens, and dual-channel real-time imaging. All participants including the PI and Co-PIs represent an interdepartmental group of faculty who, despite diverse research interests, possess commonalities in their employed approaches and instrumentation. Foreseeable outcomes from the research activities include advancement of interdisciplinary research, training, and collaboration. This new system will offer researchers the exciting opportunity to pursue several up to date research avenues including: 1) uncovering the roles of dynamin-like proteins in endocytosis, 2) characterizing anti-inflammatory signaling networks downstream of interleukin-10, 3) revealing the role of ATAF1 in plant resistance to powdery mildew pathogens, 4) understanding the role of actin cytoskeleton reorganization during the defense responses by grapevine to powdery mildew pathogens, 5) monitoring the delivery of carbon nanotubes in cells, 6) monitoring fluorescent biosensors based on conducting polymer nanowires, 7) studying neuron-glial cell communication, 8) investigating fecal coliform growth in biofilms derived from freshwater streams, and 9) studying P2 nucleotide receptor function at the molecular level. Employment of the confocal microscopy in multiple classroom settings at the university and in the region will broaden the impact of the instrument. Training with the instrument will be incorporated into the curriculums for five of our academic departments, both in traditional classes and student independent research. Acquisition of the spinning confocal system will impact many facets of the MSU community and beyond. An immediate outcome will be the expanded investigative capacity of the major users and students. As a result, faculty in several departments will be better positioned to (i) contribute to the scientific community by way of conference presentations and peer-reviewed publications and (ii) compete for external research funding. A heightened research profile at MSU will aid in the recruitment and retention of high-quality faculty and students. Furthermore, the establishment of the proposed system will broaden interdisciplinary collaboration with local universities. Collectively, greater success in these areas will increase student research opportunities and heighten the quality of undergraduate and graduate education in the region. The availability of this instrument will also contribute greatly towards the outreach programs in the local community to educate families and motivate young people to pursue careers in science and engineering. Outcomes of the research projects will be communicated through presentations by students and faculty at regional or national meetings, in peer reviewed journal publications, and on the internet.

该奖项根据 2009 年美国复苏和再投资法案（公法 111-5）提供资助。已向密苏里州立大学 (MSU) 授予赠款，用于购买配备横河旋转共焦扫描仪和四个用于大学和该地区研究和教学的激光线。该仪器不仅使研究人员能够显着提高显微成像的质量，而且还使他们有机会执行先进的技术，例如光漂白后的荧光恢复 (FRAP)、荧光共振能量转移 (FRET)、样本的光学切片和双通道实时成像。包括 PI 和 Co-PI 在内的所有参与者都代表了一个跨部门的教师群体，尽管他们的研究兴趣不同，但在所采用的方法和仪器方面具有共性。研究活动的可预见成果包括跨学科研究、培训和合作的进步。这个新系统将为研究人员提供令人兴奋的机会，以追求几个最新的研究途径，包括：1）揭示动力蛋白样内吞作用中的作用，2）表征白细胞介素10下游的抗炎信号网络，3）揭示ATAF1 在植物抗白粉病病原体中的作用，4) 了解肌动蛋白细胞骨架重组在葡萄树对白粉病病原体的防御反应中的作用，5) 监测细胞内碳纳米管的输送，6) 监测基于导电聚合物纳米线的荧光生物传感器，7) 研究神经元-胶质细胞通讯，8) 研究淡水流生物膜中粪便大肠菌的生长，9) 研究 P2 核苷酸受体功能分子水平。在大学和该地区的多个教室环境中使用共焦显微镜将扩大该仪器的影响。使用该仪器的培训将纳入我们五个学术部门的课程中，包括传统课程和学生独立研究。 获得旋转共焦系统将影响密歇根州立大学社区及其他社区的许多方面。直接的结果将是扩大主要用户和学生的调查能力。因此，多个系的教师将能够更好地（i）通过会议演讲和同行评审出版物为科学界做出贡献，以及（ii）竞争外部研究资金。密歇根州立大学的研究实力的提升将有助于招募和留住高素质的教师和学生。此外，拟议系统的建立将扩大与本地大学的跨学科合作。总的来说，这些领域取得的更大成功将增加学生的研究机会，并提高该地区本科和研究生教育的质量。该工具的可用性还将极大地促进当地社区的外展计划，以教育家庭并激励年轻人从事科学和工程职业。 研究项目的成果将通过学生和教师在地区或国家会议上的演讲、同行评审的期刊出版物和互联网上进行交流。