MRI: Acquisition of Equipment to Study Biological Interactions

MRI：购买设备来研究生物相互作用

• 批准号: 0922712
• 负责人: Yvonne Gindt
• 金额: $ 19.97万
• 依托单位: Lafayette College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922712&HistoricalAwards=false
• 关键词: MRI; Acquisition; Equipment; Study; Biological

With this award from the Major Research Instrumentation (MRI) program, Yvonne M. Gindt and her colleagues H. David Husic, William H. Miles and Jennifer L. Rutherford at Lafayette College will acquire an isothermal titration calorimeter (ITC) and a stopped flow mixer and spectrometer. They will be used in a variety of projects including: 1) mapping the binding of UV-damaged DNA to photolyase and VcCry1, enzymes that repair DNA using blue-light; 2) measuring the kinetics of DNA binding to photolyase; 3) studies of carbonic anhydrase, an enzyme which enhances the efficiency for carbon dioxide utilization for photosynthesis; 4) measurement of the kinetics of the ring-chain tautomerism of gamma-hydroxybutenolides; and 5) measurement of the kinetics of two industrially important reactions, non-cryogenic lithiations and palladium-catalyzed cross-coupling reactions, to elucidate mechanistic details of the reactions.Isothermal titration calorimetry is used to determine thermodynamic properties of chemical reactions, or of the binding of small molecules to other species by measuring temperature changes when substances are mixed (titrated). A stopped-flow apparatus is similarly used for rapid mixing and monitoring of subsequent changes using spectroscopic techniques to observe the progress of a reaction. These changes provide insight on the underlying microscopic processes that are occurring, such as the energy released in a reaction or the strength of the binding between two species or the speed of a reaction. The instruments will be used in research and laboratory classes by undergraduate students. The equipment will also strengthen existing collaborations between Lafayette College and Montclair State University with a substantial enrollment of underrepresented students.

通过大型研究工具（MRI）计划的奖项，Yvonne M. Gindt和她的同事H. David Husic，William H. Miles和Lafayette College的Jennifer L. Rutherford将获得等温滴定量热量表（ITC）（ITC）和停止的流动式搅拌机和光流式搅拌器。 它们将用于各种项目中，包括：1）映射紫外线受损的DNA与光溶解酶和VCCRY1的结合，即使用蓝光修复DNA的酶； 2）测量DNA与光溶解酶结合的动力学； 3）碳酸酐酶的研究，一种酶，可提高光合作用二氧化碳利用的效率； 4）测量γ-羟基丁烯酚的环链互变异的动力学；和5）测量两种工业重要反应的动力学，非结晶岩性和palladium催化的交叉偶联反应，以阐明反应的机械细节。在化学反应中，用于确定化学反应的热力学特性，或替代小东西的结合时，用于确定其他物种的温度，用于确定其他物种的热力学特性。 类似地，使用光谱技术来快速混合和监测随后的变化，以观察反应的进展。 这些变化提供了对正在发生的基础显微镜过程的见解，例如反应中释放的能量或两个物种之间结合的强度或反应速度。 这些仪器将由本科生用于研究和实验室课程。 该设备还将加强拉斐特学院和蒙特克莱尔州立大学之间的现有合作，并大量招募了代表不足的学生。