RUI: Testing phylogenetic hypotheses within Tubulinea, a clade of free-living lobose amoebae

RUI：测试 Tubulinea（自由生活的叶状变形虫的一个进化枝）内的系统发育假设

• 批准号: 0919152
• 负责人: Laura Katz
• 金额: $ 25.2万
• 依托单位: Smith College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919152&HistoricalAwards=false
• 关键词: RUI; Testing; phylogenetic; hypotheses; within

Much remains to be learned about the biological diversity of microbial organisms. Amoebae, the subject of this proposal, have been particularly neglected because of both their small size and simple cell structures that provide few characters for comparative studies. This project relies on molecular tools to elucidate the diversity of amoebae in the group Tubulinea, with a focus on the so-called shelled amoebae in the Arcellinida. These amoebae, which are abundant in diverse habitats including ponds, streams, and many soils, are likely important players in food webs and in Carbon cycling. Project deliverables include: deposition of new cultures of free-living amoebae, characterization of numerous genes from diverse amoebae; and creation of a framework for future studies of these poorly known species.The broader impacts of this project include: 1) training undergraduates and graduate students; 2) educational outreach to local schools, including those in the underserved city of Springfield, Massachusetts; and 3) increased participation of students from underrepresented groups, including first generation college students, through a program co-founded by the principal investigator at Smith College.

关于微生物的生物多样性还有很多尚待了解。 该提案的主题是Amoebae，由于其小尺寸和简单的细胞结构提供了比较研究的特征，因此被特别忽略了。该项目依靠分子工具来阐明小管中的变形虫的多样性，重点关注Arcellinida中所谓的壳的变形虫。 这些在包括池塘，溪流和许多土壤在内的不同栖息地中丰富的变形虫可能是食物网和碳循环中的重要参与者。 项目可交付成果包括：自由生活的新文化的沉积，对各种变形虫的众多基因的表征；并创建一个框架，用于对这些鲜为人知的物种的未来研究。该项目的广泛影响包括：1）培训本科生和研究生； 2）对当地学校的教育宣传，包括在马萨诸塞州斯普林菲尔德市服务欠缺的学校； 3）通过由史密斯学院的首席调查员共同创立的计划，包括人数不足的小组（包括第一代大学生）的学生的参与增加。