CAREER: Toward the Genetics of Meiosis: Integration of Meiotic Molecular Biology and Public School Science Outreach

职业：走向减数分裂的遗传学：减数分裂分子生物学和公立学校科学推广的整合

• 批准号: 0844941
• 负责人: Jeremy Ward
• 金额: $ 87.5万
• 依托单位: Middlebury College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0844941&HistoricalAwards=false
• 关键词: CAREER; Toward; Genetics; Meiosis; Integration

Scientific ResearchThis project uses a combination of genetics, cell biology, and molecular biology to determine the structure and function of the mouse gene Cyclin B1 interacting protein 1 (Ccnb1IP1) during male and female meiosis. This research has three components: (1) to determine the complete gene and messenger RNA transcript structure for Ccnb1IP1, (2) to determine specific protein-protein interactions involving CCNB1IP1 during meiosis in mice, and (3) to identify the extent and cause of chromosomal defects in female meiosis. This research will advance knowledge across the field of meiotic biology by providing a new mouse resource for study, by elucidating the protein networks necessary for progression through meiosis, and by determining the key constituents of female meiosis. This work represents an important contribution to the research community because it increases the understanding of meiosis, a process of central importance for the generation of life's diversity through sexual reproduction.Broader ImpactsThis project describes a program to conduct state-of-the-art molecular biological research and to integrate that research into the curricula of local public schools. An outreach program will be developed and staffed by Middlebury College undergraduate students, using a mobile DNA lab called the Gene Wagon, as an integral part of the undergraduate research program. Students will travel to local schools and, in collaboration with local teachers, will design and implement genetics-based educational laboratories. Additionally, this project will engage public school teachers through direct participation in primary research each summer. This project will improve interest and learning in biology in a school district with high rates of students below the poverty level. The benefits to society of this project are that basic research in meiosis will provide fundamental information regarding how organisms reproduce, generate genetic diversity, and as a result how they evolve. Further the principal investigator will be preparing teachers and students to succeed as scientists. The community outreach program will directly affect all local students but will be particularly beneficial to the rural poor. As a model, this program will also serve to stimulate the implementation of other such outreach programs in the area.

科学研究该项目结合遗传学、细胞生物学和分子生物学来确定小鼠基因 Cyclin B1 相互作用蛋白 1 (Ccnb1IP1) 在雄性和雌性减数分裂过程中的结构和功能。这项研究由三个部分组成：(1) 确定 Ccnb1IP1 的完整基因和信使 RNA 转录本结构，(2) 确定小鼠减数分裂过程中涉及 CCNB1IP1 的特定蛋白质-蛋白质相互作用，(3) 确定减数分裂过程中 CCNB1IP1 的具体蛋白质-蛋白质相互作用，以及 (3) 确定女性减数分裂中的染色体缺陷。这项研究将通过提供新的小鼠研究资源、阐明减数分裂进展所需的蛋白质网络以及确定雌性减数分裂的关键成分，从而推进减数分裂生物学领域的知识。这项工作对研究界做出了重要贡献，因为它增加了对减数分裂的理解，减数分裂是通过有性生殖产生生命多样性的一个至关重要的过程。更广泛的影响该项目描述了一个进行最先进的分子生物学的计划研究并将该研究纳入当地公立学校的课程。外展计划将由米德尔伯里学院的本科生开发并配备，使用名为 Gene Wagon 的移动 DNA 实验室，作为本科生研究计划的组成部分。 学生将前往当地学校，并与当地教师合作，设计和实施基于遗传学的教育实验室。 此外，该项目将通过每年夏天直接参与小学研究的方式吸引公立学校教师参与。 该项目将提高贫困线以下学生比例较高的学区对生物学的兴趣和学习。该项目对社会的好处是，减数分裂的基础研究将提供有关生物体如何繁殖、产生遗传多样性及其如何进化的基本信息。此外，首席研究员还将帮助教师和学生做好成为成功科学家的准备。 社区外展计划将直接影响所有当地学生，但对农村贫困人口尤其有利。 作为一个模型，该计划还将有助于刺激该地区其他此类外展计划的实施。