Purchase of Equipment for Polypeptide Synthesis, Purification, and Analysis

购置多肽合成、纯化、分析设备

• 批准号: 0840516
• 负责人: Andrew Maverick
• 金额: $ 10.54万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0840516&HistoricalAwards=false
• 关键词: Purchase; Equipment; Polypeptide; Synthesis; Purification

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With support from the Chemistry Research Instrumentation and Facilities: Multiuser program (CRIF:MU), the Department of Chemistry at Louisiana State University will acquire a Circular Dichroism spectrometer system. The spectrometer will be housed in the LSU protein facility and will be used by faculty at LSU and regional institutions to characterize protein structure. Some of the studies to be carried out include: (1) protein folding of DNA polymerases; (2) the study of peptides and proteins for inhibition of protein-protein interactions; (3) the synthesis and characterization of complex peptides containing post-translationally modified amino acids; (4) biophysical and structural investigations of glycoproteins; (5) designed peptides for biology and biomaterials; and (6) the crystallographic analysis of inhibitors bound to acetyl-CoA carboxylase. Circular Dichroism spectrometers, like the one purchased under this award, allow chemists to probe the secondary and higher structures in large biomolecules. Insight into the structure of these complex molecules helps scientists to understand the way that these molecules function in living systems, including humans. Besides the broader scientific impact of the science enabled by this equipment, the spectrometer will be used to train a very diverse group of young scientists at Louisiana State University.

该奖项由 2009 年美国复苏和再投资法案（公法 111-5）资助。在化学研究仪器和设施：多用户计划 (CRIF:MU) 的支持下，路易斯安那州立大学化学系将获得圆二色性光谱仪系统。该光谱仪将安装在路易斯安那州立大学蛋白质设施中，供路易斯安那州立大学和地区机构的教职员工用来表征蛋白质结构。 即将开展的一些研究包括：（1）DNA聚合酶的蛋白质折叠； (2) 抑制蛋白质-蛋白质相互作用的肽和蛋白质的研究； (3) 含有翻译后修饰氨基酸的复合肽的合成和表征； (4)糖蛋白的生物物理和结构研究； (5) 设计用于生物学和生物材料的肽； (6)与乙酰辅酶A羧化酶结合的抑制剂的晶体学分析。圆二色性光谱仪，就像根据该奖项购买的光谱仪一样，可以让化学家探测大生物分子的二级和高级结构。 深入了解这些复杂分子的结构有助于科学家了解这些分子在包括人类在内的生命系统中发挥作用的方式。 除了该设备所带来的更广泛的科学影响之外，该光谱仪还将用于培训路易斯安那州立大学一群非常多样化的年轻科学家。