SGER: Adenovirus UV Treatment - Exploration of fundamental mechanisms of disinfection

SGER：腺病毒紫外线处理——消毒基本机制的探索

• 批准号: 0832338
• 负责人: Karl Linden
• 金额: --
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0832338&HistoricalAwards=false
• 关键词: SGER; Adenovirus; UV; Treatment; Exploration

CBET-0832338K. LindenControl of viruses in drinking water is critical for public health, and disinfection is the primary barrier against disease-causing microorganisms. UV disinfection is now the method of choice for wastewater and is becoming a very important tool for disinfection of large and small drinking water systems due to the concerns over chlorination byproducts and the need to inactivate Cryptosporidium. Cell culture infectivity data generated over the past 10 years on UV treatment of adenoviruses using 254 nm low-pressure (LP) UV disinfection was used to set 2006 US EPA standards for disinfection requirements of all viruses at a level almost 5 times the typical UV dose of 40 mJ/cm2 used in practice. However, recent research by the PI and various co-investigators has found that use of newer polychromatic UV sources (medium pressure [MP] and pulsed UV) significantly improves the UV disinfection of adenoviruses. These differences between UV sources indicate that a fundamental understanding of how UV irradiation affects adenoviruses is lacking. Numerous authors have called for an increased understanding of the fundamental molecular mechanisms involved in viral response to UV as well as molecular methods for accurate pathogen detection. This research will enhance the understanding of the mechanisms behind UV disinfection of viruses and methods used will provide powerful tools for further disinfection investigations with important positive results for protection of public health.

CBET-0832338K。林登控制饮用水中的病毒对于公共卫生至关重要，而消毒是抵御致病微生物的主要屏障。紫外线消毒现在是废水处理的首选方法，并且由于对氯化副产物的担忧以及灭活隐孢子虫的需要，它正在成为大型和小型饮用水系统消毒的非常重要的工具。过去 10 年中使用 254 nm 低压 (LP) 紫外线消毒对腺病毒进行紫外线处理所产生的细胞培养物感染性数据被用来制定 2006 年美国 EPA 标准，对所有病毒进行消毒要求，其水平几乎是典型紫外线剂量的 5 倍实际使用的功率为 40 mJ/cm2。然而，PI 和各合作研究人员最近的研究发现，使用新型多色紫外线源（中压 [MP] 和脉冲紫外线）可显着改善腺病毒的紫外线消毒效果。紫外线源之间的这些差异表明，人们缺乏对紫外线照射如何影响腺病毒的基本了解。许多作者呼吁加深对病毒对紫外线反应的基本分子机制以及准确病原体检测的分子方法的了解。这项研究将加深对紫外线消毒病毒背后机制的理解，所使用的方法将为进一步的消毒研究提供有力的工具，为保护公众健康带来重要的积极成果。