SGER: Adaptation of a chromatographic technique for accurate, rapid and affordable determination of soil amino acid concentration and their fingerprints

SGER：采用色谱技术准确、快速且经济实惠地测定土壤氨基酸浓度及其指纹

• 批准号: 0818725
• 负责人: Hormoz BassiriRad
• 金额: --
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0818725&HistoricalAwards=false
• 关键词: SGER; Adaptation; chromatographic; technique; accurate

There is growing albeit circumstantial evidence that many native soils are characterized by a relatively large concentration of soluble amino acids (AAs). Additionally, numerous plant species from multitudes of ecosystems have been shown to be able to absorb AAs. Despite such evidence, there is considerable uncertainty about the relative importance of AAs to the annual N requirements of native plants. The resolution to this uncertainty is slow in coming largely due to the lack of user friendly and cost effective techniques that can be widely used in assessing soil AAs and their fingerprints in native soils. In this study, we propose to use a recently developed ion exchange column to assay AA composition/fingerprints and to measure their response to climatic and edaphic factors. Given the unequivocal fact that AAs differ significantly in their biochemical properties, it follows that each AA requires a distinct membrane bound carrier to facilitate its uptake and transport. Therefore, it is critical to know not only total availability of soil AAs but also their composition. We will use recent advances in High Performance Liquid Chromatography (HPLC) to develop rapid, accurate and affordable measurements of soil AA profiles. The proposed research should make what is currently a specialized analytical chemistry technique easily available for wide spread use in biogeochemistry research. Ultimately, we hope the results generated here will remove technical obstacles that are currently slowing down our ability to characterize and integrate soil AAs information into models of the terrestrial N cycle.

越来越多的间接证据表明，许多原生土壤的特点是含有相对较高浓度的可溶性氨基酸 (AA)。此外，来自多个生态系统的许多植物物种已被证明能够吸收氨基酸。尽管有这些证据，但氨基酸对本土植物年氮需求的相对重要性仍存在相当大的不确定性。这种不确定性的解决进展缓慢，主要是由于缺乏可广泛用于评估土壤氨基酸及其在原生土壤中的指纹的用户友好且具有成本效益的技术。 在这项研究中，我们建议使用最近开发的离子交换柱来测定 AA 成分/指纹，并测量它们对气候和土壤因素的反应。鉴于 AA 的生化特性存在显着差异这一明确事实，因此每种 AA 都需要不同的膜结合载体来促进其摄取和运输。因此，不仅了解土壤氨基酸的总有效性，而且了解它们的组成也至关重要。我们将利用高效液相色谱 (HPLC) 的最新进展来开发快速、准确且经济实惠的土壤 AA 谱测量方法。拟议的研究应该使目前的专业分析化学技术能够轻松地在生物地球化学研究中广泛使用。最终，我们希望这里产生的结果将消除目前阻碍我们表征土壤氨基酸信息并将其整合到陆地氮循环模型中的技术障碍。