Ultra-Efficient Plasmonic Nanoparticle Markers for Second Harmonic Imaging Microscopy

用于二次谐波成像显微镜的超高效等离子体纳米颗粒标记

• 批准号: 0756693
• 负责人: Hans Robinson
• 金额: $ 30万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0756693&HistoricalAwards=false
• 关键词: Ultra; Efficient; Plasmonic; Nanoparticle; Markers

CBET-0756693, RobinsonThe intent of this proposal is to develop and characterize a new class of nanoparticles designed to operate as ultra-bright markers for second harmonic imaging microscopy (SHIM) and potentially for other nonlinear microscopy techniques. In order to accomplish this goal the investigators have established two objectives; the first objective is to fabricate and characterize several different types of SHIM enhanced nanoparticles using both lithographic and chemical synthesis techniques to give maximum flexibility. The second is to actually demonstrate SHIM imaging and hyperthermia in a biological setting. In order to accomplish this aim, the investigators will use bifunctional crosslinkers to apply a protective coating to the particles, making them biologically stable and non-toxic, and also easy to conjugate with an antibody or ligand (such as folic acid) to promote selective binding of the particles to target cells and tissues. The investigators will demonstrate the biocompatibility of the nanoparticles as well as their potential to perform targeted therapy through hyperthermia.

CBET-0756693，Robinson的意图是开发和表征一类新的纳米颗粒，旨在用作第二次谐波显微镜（SHIM）的超亮标记，并可能用于其他非线性显微镜技术。为了实现这一目标，调查人员已经建立了两个目标。第一个目的是使用光刻和化学合成技术来制造和表征几种不同类型的SHIM垫片增强的纳米颗粒，以提供最大的柔韧性。第二个是在生物学环境中实际证明垫片成像和高温。为了实现这一目标，研究人员将使用双功能交联剂将保护性涂层施加到颗粒上，使其在生物学上稳定且无毒，并且易于与抗体或配体（例如叶酸）（例如叶酸）偶联以促进颗粒与靶细胞和组织的选择性结合。研究人员将证明纳米颗粒的生物相容性以及通过高温进行靶向治疗的潜力。