Arabidopsis TSO1 in Transcription and Chromatin Function

拟南芥 TSO1 的转录和染色质功能

• 批准号: 0744752
• 负责人: Zhongchi Liu
• 金额: --
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0744752&HistoricalAwards=false
• 关键词: Arabidopsis; TSO1; Transcription; Chromatin; Function

During the embryonic and postembryonic development of plants and animals, cell proliferation and cell differentiation must be coordinated to achieve final organ size, shape and function. It appears that plants and animals utilize distinct strategies to achieve this coordinated cell growth. Plant cells are particularly flexible in that they can be easily cultured in medium and regenerated into an entirely new plant. It was proposed that certain coordination genes, which function differently in plants, might underlie this distinct property of plant cells. Previous genetic analyses of an Arabidopsis thaliana mutant tso1 showed a failure of cell differentiation in the mutant flowers, suggesting that the TSO1 gene might encode such an important coordinator gene. The sequence similarity between the Arabidopsis TSO1 protein and the Drosophila Mip120 protein suggested that TSO1 might function as a crucial component of a chromatin complex. This oneyear pilot project is aimed at establishing several essential tools and reagents necessary for the characterization of TSO1 and testing the hypothesis that TSO1 is a component of a novel plant chromatin complex. These studies may shed light on the unique properties of plant cells in tissue and whole plant regeneration. Due to the conservation of genetic mechanisms between Arabidopsis and other plants, the work will also advance research and application in important crop plants. The project will involve and train Ph.D. students, undergraduate students, and postdoctoral fellows. Efforts will be placed on recruiting students from under representative groups at the University of Maryland and nearby universities.

在植物和动物的胚胎和胚胎后发育期间，必须协调细胞增殖和细胞分化，以实现最终器官的大小，形状和功能。似乎动植物利用不同的策略来实现这种协调的细胞生长。植物细胞特别灵活，因为它们很容易在培养基中培养并再生为全新的植物。有人提出，某些在植物中起作用不同的协调基因可能是植物细胞的这种独特特性的基础。先前对拟南芥突变体TSO1的遗传分析显示突变花中细胞分化的失败，这表明TSO1基因可能编码如此重要的协调基因。拟南芥TSO1蛋白与果蝇MIP120蛋白之间的序列相似性表明，TSO1可能是染色质复合物的关键成分。这个年度试点项目旨在建立表征TSO1所需的几种必需工具和试剂，并检验了TSO1是新型植物染色质复合物的组成部分的假设。这些研究可能会阐明植物细胞在组织和整个植物再生中的独特特性。由于拟南芥与其他植物之间的遗传机制保存，这项工作还将推动重要的作物植物的研究和应用。该项目将涉及并培训博士学位。学生，本科生和博士后研究员。将努力为马里兰州和附近大学的代表团体的招募学生做出努力。