Collaborative Research: Integrated Microsystem for Ultrasensitive Airborne Pathogen Detection in Real Time

合作研究：实时超灵敏空气传播病原体检测的集成微系统

• 批准号: 0725525
• 负责人: Sung Cho
• 金额: $ 16万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-11-01 至 2011-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0725525&HistoricalAwards=false
• 关键词: Collaborative; Research; Integrated; Microsystem; Ultrasensitive

Collaborative Research: Integrated Microsystem for Ultrasensitive Airborne Pathogen Detection in Real TimeThe objective of this research is to develop an airborne pathogen sensing system, capable of fast and sensitive environmental monitoring, for use in a broad range of infrastructures. The approach is to develop both microfluidic operations for sample collection and preparation and quantum dots-based nanosensors for identification of pathogens according to their ribosomal RNA sequences. The microfluidic unit and the nanosensor unit will be integrated into a system whose performance will be exemplified through analysis of Bacillus spores. The use of the droplet-based microfluidic operations will minimize the requirement of liquid volume and avoid the need for building moving components on the chip for sample processing that both reduces the consumption of biochemical reagents and the complexity in constructing an integrated microfluidic processing system. The highly sensitive rRNA nanosensors will eliminate the need for polymerase chain reaction (PCR) amplification, thereby simplifying assay protocols and system integration. This sensing system will have broader impacts with the societal benefits including (i) immediate detection of bio-warfare pathogens such as anthrax to prevent their possible catastrophe and (ii) surveillance of environments in, for example, transportation infrastructures, healthcare buildings, and workplaces. Besides, this research will also have significant impacts on education by (i) establishing micro/nano scientific visual library on web based on the research outcome; (ii) developing micro/nano science coursework with a lab session; and (iii) having undergraduates especially from the underrepresented groups to participate in the research activities.

合作研究：实时超灵敏空气传播病原体检测的集成微系统这项研究的目标是开发一种空气传播病原体传感系统，能够快速、灵敏地监测环境，适用于广泛的基础设施。该方法旨在开发用于样品收集和制备的微流体操作，以及用于根据病原体的核糖体 RNA 序列识别病原体的基于量子点的纳米传感器。微流体单元和纳米传感器单元将集成到一个系统中，该系统的性能将通过芽孢杆菌孢子的分析来验证。使用基于液滴的微流控操作将最大限度地减少液体体积的需求，并避免在芯片上构建用于样品处理的移动组件的需要，这既减少了生化试剂的消耗，又降低了构建集成微流控处理系统的复杂性。高灵敏度的 rRNA 纳米传感器将消除聚合酶链式反应 (PCR) 扩增的需要，从而简化检测方案和系统集成。该传感系统将产生更广泛的影响和社会效益，包括（i）立即检测炭疽等生物战病原体，以防止其可能发生的灾难；（ii）对交通基础设施、医疗建筑和工作场所等环境进行监测。此外，本研究还将对教育产生重大影响：（i）根据研究成果在网络上建立微纳米科学视觉图书馆； (ii) 通过实验室课程开发微/纳米科学课程； (iii) 让本科生，特别是来自弱势群体的本科生参与研究活动。