Collaborative Research: Cohesive Strength and Detachment of Bacterial Biofilms

合作研究：细菌生物膜的内聚强度和分离

• 批准号: 0728550
• 负责人: Raymond Hozalski
• 金额: $ 21.8万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0728550&HistoricalAwards=false
• 关键词: Collaborative; Research; Cohesive; Strength; Detachment

Holzalski, Raymond, PIUniversity of Minnesota-Twin CitiesCBET-0728550Stewart, Phillip, PIMontana State UniversityCBET-0728621Collaborative Research: Cohesive Strength and Detachment of Bacterial BiofilmsThe goal of this project is to develop effective strategies for controlling and removing microbial biofilms based on an improved understanding of the mechanisms of cohesion of the biofilm extracellular matrix. Biofilms are dense agglomerations of bacterial or fungal cells that attach to wetted surfaces. These cells are held together by a mixture of highly hydrated biopolymers that the cells themselves secrete. Biofilms are responsible for troublesome fouling in water distribution systems, industrial equipment and persistent infections in medicine and dentistry. This project shifts the approach to controlling unwanted biofilms from killing microorganisms with antimicrobial agents to weakening the biofilm structure and promoting detachment. A key to this strategy is to directly measure the cohesive strength of biofilms and relate strength to detachment. Specific objectives of this project are to: (1) investigate mechanisms responsible for biofilm cohesive strength and detachment, (2) evaluate the effect of environmental conditions on strength and detachment, and (3) measure the effects of potential disruptor compounds on strength and detachment. The objectives will be addressed experimentally in a combination of single-species and mixed-species biofilms. A central technique is a micro-cantilever method for pulling on a piece of biofilm and quantifying its mechanical properties. Other methods include time-lapse imaging of biofilm structures in glass capillary reactors under continuous-flow conditions by light microscopy and confocal scanning laser microscopy and measurements of biofilm detachment in rotating disk reactors.The research will impact engineering through the development of methods to control biofouling, such as in pipelines, where decreased biofilm accumulation could improve the safety of water supplies and reduce energy losses. In terms of public health, this research will provide alternative methods for fighting bacterial infections, such as those encountered on medical implant devices or in the lungs of cystic fibrosis patients, to reduce the need for redundant surgeries and improve/extend life. Graduate and undergraduate students from both institutions will participate in the research. Both groups of students will be afforded opportunities to interact with industry and to present to industrial associates of the Center for Biofilm Engineering at the biannual conference hosted by the center.

Holzalski，Raymond，明尼苏达州TWIN COITSCBET-0728550 Stertewart，Phillip，Pimontana State Universitycbet-07286211核算研究：凝聚力的研究：基于策略的基于策略的基于策略的目标，该计划是基于策略的，以控制有效的机构，该机构是基于策略的，该机构是基于机构的，该策略是基于机构的，该机构是基于机构的，该机构是基于策略的，生物膜外基质的内聚力。生物膜是附着在湿表面的细菌或真菌细胞的致密聚集。这些细胞由细胞本身分泌的高度水合生物聚合物的混合物结合在一起。生物膜负责在水分配系统，工业设备以及医学和牙科中的持续感染中造成麻烦。该项目将控制不良生物膜的方法从用抗菌剂杀死微生物到削弱生物膜结构并促进脱离的方法。该策略的关键是直接测量生物膜的凝聚力，并将强度与脱离相关。该项目的具体目标是：（1）调查负责生物膜内聚力强度和脱离的机制，（2）评估环境条件对强度和脱离的影响，以及（3）测量潜在破坏者化合物对强度和脱离的影响。这些目标将通过单物种和混合物种生物膜的结合进行实验解决。一种中央技术是一种微型委托人方法，用于拉动一块生物膜并量化其机械性能。其他方法包括通过光学显微镜和共同扫描激光显微镜在连续流条件下对生物膜结构的延时成像以及旋转磁盘反应器中生物膜脱离的测量。该研究将通过控制生物源的方法来降低流水量的生物效应，从而降低了流入量的生物效应。在公共卫生方面，这项研究将提供替代细菌感染的替代方法，例如在医疗植入器设备上或囊性纤维化患者肺部遇到的方法，以减少对冗余手术的需求并改善/延长寿命。来自两个机构的研究生和本科生将参与研究。两组学生都将获得与行业互动的机会，并在该中心举办的双年度会议上向生物膜工程中心的工业伙伴介绍。