Collaborative Research: Evolutionary and Ecological Physiology of Blue Mussels (genus Mytilus): Gene and Protein Expression and Molecular Evolution in Differently Adapted Congeners

合作研究：蓝贻贝（贻贝属）的进化和生态生理学：不同适应同类中的基因和蛋白质表达以及分子进化

• 批准号: 0717087
• 负责人: Lars Tomanek
• 金额: --
• 依托单位: California Polytechnic State University Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0717087&HistoricalAwards=false
• 关键词: Collaborative; Research; Evolutionary; Ecological; Physiology

The distribution patterns and sensitivities to climate change of different species will be affected by their tolerance of environmental stress. This project will characterize the physiological processes in native and invasive species of blue mussels (genus Mytilus) that are most critical in the animal''s responses to changes in temperature and salinity, on a scale from minutes to weeks and even to generations. This work is the first application of modern genome-enabled technologies (transcriptomics - the study of gene expression, and proteomics - identification of proteins present in a cell) to describe the dynamics of thousands of genes and proteins simultaneously in marine animals subjected to environmental stress. In addition, this project will compare gene sequences and protein structure and function to identify the characteristics of proteins that determine the species'' different tolerance limits. This project has many broader impacts. These studies will examine why an invasive blue mussel species from the Mediterranean Sea has successfully replaced the native blue mussel along the southern portion of the Pacific Coast of North America. The blue mussels to be studied are of great interest to researchers in ecology, marine science, and evolutionary biology, as well as to State and Federal natural resource managers who seek to understand the environmental impacts of climate change on natural populations and the processes by which invasive species succeed in new habitats. The results will be of special significance to the "Mussel Watch" Project, which uses mussels to monitor the water quality status of coastal and estuarine waters. The collaboration between Stanford University and California Polytechnic State University, San Luis Obispo, brings together students from all stages of training, from high school students to postdoctoral researchers, in an effort to foster a socially and ethnically diverse research environment. Cross-campus exchanges will facilitate an excellent learning environment for students at both campuses.

不同物种的分布格局和对气候变化的敏感性会受到其对环境胁迫的耐受力的影响。 该项目将描述本地和入侵的蓝贻贝（贻贝属）物种的生理过程，这些过程对于动物对温度和盐度变化的反应至关重要，范围从几分钟到几周甚至几代。 这项工作是现代基因组技术（转录组学 - 研究基因表达和蛋白质组学 - 识别细胞中存在的蛋白质）的首次应用，以描述遭受环境压力的海洋动物中同时存在的数千个基因和蛋白质的动态。 此外，该项目还将比较基因序列和蛋白质结构和功能，以确定决定物种不同耐受限度的蛋白质特征。 该项目具有许多更广泛的影响。 这些研究将探讨为什么来自地中海的入侵蓝贻贝物种成功取代了北美太平洋海岸南部的本地蓝贻贝。 生态学、海洋科学和进化生物学的研究人员以及寻求了解气候变化对自然种群的环境影响及其过程的州和联邦自然资源管理者对要研究的蓝贻贝非常感兴趣。入侵物种在新的栖息地取得成功。 该研究结果对于“贻贝观察”项目具有特殊意义，该项目利用贻贝监测沿海和河口水域的水质状况。 斯坦福大学和加州理工州立大学圣路易斯奥比斯波分校之间的合作，汇集了从高中生到博士后研究人员等各个培训阶段的学生，努力营造一个社会和种族多元化的研究环境。 跨校区交流将为两个校区的学生提供良好的学习环境。