RUI: Model Compounds for P460 from Hydroxylamine Oxidoreductase

RUI：羟胺氧化还原酶 P460 的模型化合物

• 批准号: 0718749
• 负责人: Edward Zovinka
• 金额: $ 9.5万
• 依托单位: Saint Francis University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718749&HistoricalAwards=false
• 关键词: RUI; Model; Compounds; P460; Hydroxylamine

Professor Edward P. Zovinka, Department of Chemistry, Mathematics, and Physical Science, Saint Francis University is supported by the Inorganic, Bioinorganic and Organometallic Chemistry Program to study models for hydroxylamine oxidoreductase (HAO) in Nitrosomonas europea (Ne), an autotrophic soil bacteria that oxidizes nitrogenous materials in order to make energy. HAO contains a unique tyrosine-porphyrin connection that has been speculated to play an important role in the enzyme''s activity. To better understand the chemistry of the active site, undergraduate researchers synthesize and study model compounds containing meso-(hydroxyphenyl)porphyrin ligands as analogs for the porphyrin-tyrosine moiety. The porphyrin ligands are coordinated to biologically significant iron and the reactivities of the compounds with hydroxylamine are studied. The group utilizes Green Chemistry principles in the synthesis of the ligands. This includes the use of microwave methods to improve reaction yields and decrease reaction times. Scientific ethics are stressed to all participating undergraduate researchers through literature discussions.

爱德华·佐维卡（Edward P.这将氮材料氧化以制造能量。 HAO包含独特的酪氨酸 - 孢子蛋白连接，据推测，该连接在酶的活性中起着重要作用。 为了更好地了解活性位点的化学性质，本科研究人员合成和研究含有中（羟基苯基）卟啉配体的化合物作为卟啉 - 酪氨酸部分的类似物。 卟啉配体与生物学上具有重要意义的铁协调，并研究了化合物与羟胺的反应性。 该小组在配体的合成中利用绿色化学原理。 这包括使用微波方法来提高反应产量并减少反应时间。 通过文献讨论，所有参与的本科研究人员都强调了科学伦理。