Photodissociation for Ion Trap Mass Spectrometry

离子阱质谱的光解离

• 批准号: 0718320
• 负责人: Jennifer Brodbelt
• 金额: $ 47.5万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718320&HistoricalAwards=false
• 关键词: Photodissociation; Ion; Trap; Mass; Spectrometry

The Analytical and Surface Chemistry program in the Division of Chemistry will support the research program of Prof. Jennifer Broadbelt of the University of Texas at Austin. Prof. Broadbelt's research program in the area of mass spectrometry will involve the use of both infrared and ultraviolet lasers for photo dissociation of ions in a quadrupole ion trap mass spectrometer, along with novel chemical derivatization methods to add chemical selectivity or enhance absorptivities of molecules. Upon irradiation of ions with infrared or ultraviolet photons, the ions absorb energy that leads to bond cleavages, resulting in formation of diagnostic fragment ions. These fragmentation patterns provide a "fingerprint" of the molecules, thus allowing confident identification of peptides and other biologically relevant molecules. The absorption of photons is essential for the success of this analytical method, and thus the absorptivity of molecules can be modulated by attaching different chemical groups, termed "chromophores", that enhance the absorption of photons. The objective of the study is to increase the photon absorption efficiencies of targeted analytes by appending chromophores via an array of derivatization strategies. Prof. Broadbelt and her students will append chemical groups to biomolecules that promote photoactivated reactions in the gas phase, such as the selective cleavage of polypeptide backbones in a site-specific, predictable manner upon photoactivation. The potential benefits of the proposed work to society at large include the training and education of future scientists, especially in the area of biological mass spectrometry, the development of innovative laser-based methods for structural characterization of biological molecules, and implementation of new ion activation strategies that are expected to be important in mass spectrometric analysis in many areas, ranging from proteomics to understanding the complexities of interactions of biological macromolecules.

化学系的分析和表面化学计划将支持德克萨斯大学奥斯汀分校詹妮弗·布罗德贝尔特教授的研究计划。 Broadbelt教授在质谱法领域的研究计划将涉及使用红外和紫外线激光器在四极离子陷阱质谱仪中拍摄离子，以及新型的化学衍生化方法，以添加化学选择性或增强分子的吸收性。 在用红外或紫外光子照射离子后，离子吸收的能量会导致键裂解，从而形成诊断片段离子。 这些碎裂模式提供了分子的“指纹”，从而可以自信地鉴定肽和其他与生物学相关的分子。 光子的吸收对于这种分析方法的成功至关重要，因此可以通过连接不同化学基团的不同化学基团的吸收性来调节，称为“发色团”，从而增强了光子的吸收。该研究的目的是通过通过一系列衍生化策略附加发色团来提高目标分析物的光子吸收效率。 Broadbelt教授和她的学生将将化学基团附加到促进气相中光活化反应的生物分子上，例如在光活化后以特定于位点特异性的，可预测的方式对多肽骨架的选择性切割。 拟议的工作对整个社会的潜在好处包括对未来科学家的培训和教育，尤其是在生物学质谱学领域，开发基于创新激光的生物分子结构表征的创新激光方法，以及新离子激活策略的实施，这些方法在许多领域的质谱分析中有望在蛋白质相互作用的质谱分析中变得重要，这些方法在质谱分析中很重要。