Functionalization of Polymer Brushes for Selective Capture and Analysis of Phosphorylated and Methionine-Containing Peptides

聚合物刷的功能化用于磷酸化和含蛋氨酸肽的选择性捕获和分析

基本信息

批准号：
0616795
负责人：
Merlin Bruening
金额：
$ 37.5万
依托单位：
Michigan State University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-01 至 2010-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0616795&HistoricalAwards=false
关键词：
Functionalization Polymer Brushes Selective Capture

项目摘要

ABSTRACT CHE-0616795Bruening/Michigan StateWith the support of the Analytical and Surface Chemistry Program, the group of Professor Merlin Bruening at Michigan State University is developing methods for selectively capturing proteins and peptides for analysis by Matrix-Assisted Laser Desorption/Ionization mass spectrometry. The biomolecules are captured directly on the plate used for sample introduction, avoiding time-consuming separation techniques and associated sample loss. Specifically, controlled growth of polymer brushes on the sample probe and derivatization of these brushes with Fe(III) complexes will be tested for enabling selective binding and highly sensitive analysis of phosphorylated peptides. Methods for selective capture of methionine-containing peptides on sample plates will also be developed. Control over the density, thickness, composition, and swelling of polymer brushes on sample plates will be vital for achieving low detection limits in both areas. Identification and quantitation of protein phosphorylation is vital for understanding processes such as cell regulation and diseases such as cancer. Selective binding of the methionine-containing peptides will decrease the number of signals from a digest mixture but still provide effective protein coverage. This is expected to greatly simplify the analysis of complex biological samples. Work on this project will be performed by students at all levels including high school, undergraduate, and graduate students, providing a broad perspective on important biochemical and analytical research areas.

摘要CHE-0616795繁殖/密歇根州州立大学的支持和表面化学计划的支持，密歇根州立大学的Merlin Bruening教授正在开发用于选择性捕获蛋白质和肽的方法，以通过矩阵辅助的激光吸收/离子化质量图进行分析。将生物分子直接捕获在用于样品引入的板上，避免了耗时的分离技术和相关的样品损失。具体而言，将测试聚合物刷在样品探针上的受控生长，并将这些用Fe（III）络合物衍生化，以实现对磷酸化肽的选择性结合和高度敏感的分析。还将开发用于选择性捕获样品板上含蛋氨酸的肽的方法。控制样品板上聚合物刷的密度，厚度，成分和肿胀对于在两个区域中达到低检测极限至关重要。蛋白质磷酸化的鉴定和定量对于理解诸如细胞调节和癌症等疾病之类的过程至关重要。含蛋氨酸的肽的选择性结合将减少来自摘要混合物的信号数量，但仍提供有效的蛋白质覆盖率。预计这将大大简化复杂生物样品的分析。该项目的工作将由包括高中，本科生和研究生在内的各个级别的学生进行，并为重要的生化和分析研究领域提供广泛的看法。